主管：中华人民共和国司法部
主办：司法鉴定科学研究院
ISSN 1671-2072  CN 31-1863/N
中国司法鉴定

中国司法鉴定 ›› 2010 ›› Issue (6): 21-26.

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LC-MS/MS 法测定蓝色圆珠笔油墨中的碱性染料

孙其然，罗仪文，徐 彻   

  1. 上海市法医学重点实验室 司法部司法鉴定科学技术研究所，上海 200063
  • 收稿日期:2010-07-18 出版日期:2010-11-15 发布日期:2022-07-07
  • 通讯作者: 徐彻（（1965-）男，高级工程师。E-mail∶xuc@ssidcn。
  • 作者简介:孙其然（1984-），女，助理工程师，主要从事微量物证tion monitoring，MRM）下，只选择目标物特征性的母鉴定工作。E-mail∶sunqiran1984@yahoo.com.cn。
  • 基金资助:
    科研院所公益专项（GY0708、GY0808）

Determination of Basic Dyes in Blue Ballpoint Pen Ink by Liquid Chromatography Tandem Mass Spectrometry

SUN Qi-ran, LUO Yi-wen, XU Che   

  1. Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, Shanghai 200063, China
  • Received:2010-07-18 Published:2010-11-15 Online:2022-07-07

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摘要： 目的 建立蓝色圆珠笔油墨中碱性染料的LC-MS/MS方法.为蓝色圆珠笔油墨的种类鉴别提供方法。方法 用二级质谱寻找并确定结晶紫、甲基紫、维多利亚蓝 B、碱性紫 14、碱性蓝7和罗丹明 B等碱性染料的特征性母离子/子离子对。收集 50种蓝色圆珠笔，划线后对其笔道用 0.5mm 直径打孔器取样，乙腈超声提取。液相分离采用Waters XBridge C8 柱，流动相为0.1%甲酸缓冲液（A）-乙腈（B），梯度程序洗脱。结果 4个点的取样量足以满足检测需要，采用相对峰面积的定量方法，结果重现性好，RSD%≤2.3%。应用该方法对50种蓝色圆珠笔油墨中的碱性染料进行检测，区分率为94.4%。结论 所建 LC-MS/MS方法定性准确，定量可靠，为蓝色圆珠笔油墨的种类鉴别提供了方法。

关键词: 蓝色圆珠笔油墨, 碱性染料, LC-MS/MS, 种类鉴别

Abstract: Objective To establish a method of analysing of basic dyes in blue ballpoint pen ink by liquid chromatography tan-dem mass spectrometry for the discrimination of blue ballpoint pen ink on a questioned document. Methods Specific precursor and product ion pairs of crystal violet,methyl violet, victoria blue B, basic violet 14, basic blue 7 and rhodamine B were deter-mined by a triple quadrupole tandem mass spectrometer.Ink lines of fifty blue ballpoint pens were drawn on one piece of print paper. Four hole punches of each ink sample were collected with a Harris MICRO-PUNCH (0.5 mm i.d.). Acetonitrile was added to perform extraction in an ultrasonic water bath. Waters XBridge C18 column was used for separation.Target compounds were eluted by a gradient procedure in which O.1% formic acid solution and acetonitrile were used as mobile phase. Results Four hole punches of ink samples provided good signals for the analysis. Intra-batch precision was quite good as the relative standard deviation was no more than 2.3%.Discrimination power of established method was 94.4%. Conclusion The present study provided a precise method for the discrimination of blue ballpoint pen ink on a questioned document. 

Key words: blue ballpoint pen ink, basic dye, LC-MS/MS, discrimination

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