主管:中华人民共和国司法部
主办:司法鉴定科学研究院
ISSN 1671-2072  CN 31-1863/N

中国司法鉴定 ›› 2011 ›› Issue (6): 35-37.

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应用多种STR技术对基因嵌合体进行亲权鉴定分析

林 源,赵珍敏,李 莉   

  1. 司法部司法鉴定科学技术研究所,上海市法医学重点实验室,上海 200063
  • 收稿日期:2011-03-12 出版日期:2011-11-15 发布日期:2022-07-29
  • 作者简介:林源(1964-),男,副研究员,主要从事法医遗传学研究。
  • 基金资助:
    中央级科研院所社会公益研究资助项目(GY0803)

Paternity Analysis of Gene Chimera with Multiple STR Loci

LIN Yuan, ZHAO Zhen-min, LI Li   

  1. Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, 
    Shanghai 200063,China
  • Received:2011-03-12 Published:2011-11-15 Online:2022-07-29

摘要: 目的 查找嵌合基因的来源并进行父母和孩子的亲权鉴定。方法 采用Chelex-100法抽提基因组DNA,用复合扩增和荧光检测技术对STR、X-STR和Y-STR基因座进行分型。结果 父亲为XX,XY基因嵌合体,其能够提供给孩子必须的遗传基因。结论 父亲为被检孩子的生物学父亲。

关键词:  , PCR复合扩增、荧光检测、STR、X-STR、Y-STR检测技术、基因嵌合体

Abstract: Objective To detect the source of the chimeric genes and perform paternity test with STR,X-STR and Y-STR techniques. Methods Genomic DNA was extracted by Chelex-100 method. STR, X-STR and Y-STR loci were typed by multiplex amplification and fluorescence detection. Results The gender gene of the detected father is the chimera of XX and XY. The detected father can provide the paternal gene to the detected child. Conclusion The detected father is the child's biological father. 

Key words: PCR amplification, fluorescence detection, STR, X-STR, Y-STR, gene chimera

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