电子烟油中尼秦类新精神活性物质美托尼秦的鉴定分析

doi:10.3969/j.issn.1671-2072.2025.03.007

中国司法鉴定 ›› 2025 ›› Issue (3): 51-58.DOI: 10.3969/j.issn.1671-2072.2025.03.007

电子烟油中尼秦类新精神活性物质美托尼秦的鉴定分析

杨乔¹，胡瑞²，吴波³，曹煜¹，唐泊伊²，张忠辉¹，苏梦翔²

1. 南京市公安局禁毒支队，江苏南京 210042； 2. 中国药科大学药学院，江苏南京 210009；
3.南京市公安局刑事侦查局，江苏南京 210001

收稿日期:2024-04-10 出版日期:2025-05-15 发布日期:2025-05-16
作者简介:杨乔（1987—），男，工程师，硕士，主要从事毒品分析研究。E-mail:451062477@qq.com

Identification of Metonitazene, a Nitazene New Psychoactive Substance in E-liquids

YANG Qiao¹, HU Rui², WU Bo³, CAO Yu¹, TANG Boyi², ZHANG Zhonghui¹, SU Mengxiang²

1. Division of Narcotic Control, Nanjing Public Security Bureau, Nanjing 210042, China;
2. School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China;
3. Department of Criminal Investigation, Nanjing Public Security Bureau, Nanjing 210001, China

Received:2024-04-10 Published:2025-05-15 Online:2025-05-16

摘要/Abstract

摘要： 目的对毒品案件缴获的电子烟油中的美托尼秦进行定性检验。方法采用气相色谱-质谱法（gas chromatography-mass spectrometry，GC-MS）和超高效液相色谱-高分辨质谱法（ultra-high performance liquid chromatography-high resolution mass spectrometry，UHPLC-HRMS）对美托尼秦进行鉴定，同时通过紫外可见吸收光谱法（ultraviolet-visible absorption spectrum，UV-Vis）、傅里叶变换红外光谱法（Fourier transform infrared spectroscopy，FTIR）和核磁共振波谱法（nuclear magnetic resonance spectroscopy，NMR）分析美托尼秦。结果 GC-MS结果显示，保留时间15.295 min处有一未知化合物，其特征离子为m/z 86（基峰）、121、58、382，后经标准品比对确证该未知化合物为美托尼秦，并提供美托尼秦UV-Vis、FTIR和NMR相关结构数据。结论综合运用多种技术对电子烟油检材中的美托尼秦进行分析，可为该类新精神活性物质的检验鉴定提供参考。

关键词: 美托尼秦, 气相色谱-质谱法, 超高效液相色谱-高分辨质谱法, 紫外可见吸收光谱法, 傅里叶变换红外光谱法, 核磁共振波谱法, 电子烟油

Abstract: Objective To identify metonitazene in e-liquids seized from drug cases. Methods Methods of gas chromatography-mass spectrometry (GC-MS) and ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS) were established to identify metonitazene. The compound was also analyzed by ultraviolet-visible absorption spectrum (UV-Vis), Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR) methods. Results The GC-MS result showed that there was an unknown compound at the retention time of 15.295 min, and its characteristic ions were m/z 86 (base peak), 121, 58, and 382, which was identified as metonitazene by comparing with the standard substance. The structural information of the target acquired by UV-Vis, FTIR and NMR were supplemented. Conclusion The comprehensive use of various techniques to analyze metonitazene in e-liquid samples can provide structural information to confirm the detection of this kind of new psychoactive substances.

Key words: metonitazene, gas chromatography-mass spectrometry (GC-MS), ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS), ultraviolet-visible absorption spectrum (UV-Vis), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance spectroscopy (NMR), e-liquid

中图分类号:

DF795.1

杨乔, 胡瑞, 吴波, 曹煜, 唐泊伊, 张忠辉, 苏梦翔. 电子烟油中尼秦类新精神活性物质美托尼秦的鉴定分析[J]. 中国司法鉴定, 2025(3): 51-58.

YANG Qiao, HU Rui, WU Bo, CAO Yu, TANG Boyi, ZHANG Zhonghui, SU Mengxiang. Identification of Metonitazene, a Nitazene New Psychoactive Substance in E-liquids[J]. Chinese Journal of Forensic Sciences, 2025(3): 51-58.

参考文献

[ 1 ] VANDEPUTTE M M，VAN UYTFANGHE K，LAYLE N K，et al. Synthesis，chemical characterization，and μ-opioid receptor activity assessment of the emerging group of “nitazene” 2-benzylbenzimidazole synthetic opioids[J]. ACS Chemical Neuroscience，2021，12（7）： 1241-1251.
[ 2 ] GROSS F，TURRIAN H. Über Benzimidazolderivate mit starker analgetischer Wirkung[J]. Experientia，1957，13（10）： 401-403.
[ 3 ] World Health Organization （WHO）. Critical review report： Metonitazene[EB/OL]. [2024-02-17]. https：//www.who.int/publications/m/item/metonitazene---critical-revie w-report.
[ 4 ] BLANCKAERT P, CANNAERT A, VAN UYTFANGHE K, et al. Report on a novel emerging class of highly potent benzimidazole NPS opioids：Chemical and in vitro functional characterization of isotonitazene[J]. Drug Testing and Analysis，2020，12（4）：422-430.
[ 5 ] Schedules of controlled substances： Temporary placement of butonitazene，etodesnitazene，flunitazene，metodesnitazene，metonitazene，N-pyrrolidino etonitazene，and protonitazene in schedule I[J]. Federal Register，2022，87（70）：21556-21561.
[ 6 ] MORIOKA F，FUJII S，KAMATA H，et al. Postmortem examination and toxicological analysis for acute metonitazene intoxication in Japan： A case report[J]. Legal Medicine，2023，61： 102216.
[ 7 ] MONTANARI E，MADEO G，PICHINI S，et al. Acute intoxications and fatalities associated with benzimidazole opioid （nitazene analog） use： A systematic review[J]. Therapeutic Drug Monitoring，2022，44（4）： 494-510.
[ 8 ] KROTULSKI A J，PAPSUN D M，WALTON S E，et al. Metonitazene in the United States—Forensic toxicology assessment of a potent new synthetic opioid using liquid chromatography mass spectrometry[J]. Drug Testing and Analysis，2021，13（10）： 1697-1711.
[ 9 ] BLANCKAERT P，BALCAEN M，VANHEE C，et al. Analytical characterization of “etonitazepyne，” a new pyrrolidinyl-containing 2-benzylbenzimidazole opioid sold online[J]. Drug Testing and Analysis，2021，13（9）： 1627-1634.
[10] PAPSUN D M，KROTULSKI A J，LOGAN B K. Proliferation of novel synthetic opioids in postmortem investigations after core-structure scheduling for fentanyl-related substances[J]. The American Journal of Forensic Medicine and Pathology，2022，43（4）： 315-327.
[11] PARDI J，FORD S，COOPER G. Validation of an analytical method for quantitation of metonitazene and isotonitazene in plasma，blood，urine，liver and brain and application to authentic postmortem casework in New York City[J]. Journal of Analytical Toxicology，2023，47（8）： 648-655.
[12] SCHÜLLER M，LUCIC I，ØIESTAD Å M L，et al. High-throughput quantification of emerging “nitazene” benzimidazole opioid analogs by microextraction and UHPLC-MS-MS[J]. Journal of Analytical Toxicology，2023，47（9）： 787-796.
[13] 沈瑞迪，李彭，胡文，等. 新型合成阿片类物质美托尼秦的定性检验[J]. 刑事技术，2024，49（4）： 359-366.
[14] 代茵茵，赵崐迪，王越，等. GC-MS/MS法鉴别毒品中的依托尼秦和依替氮卓[J]. 中国法医学杂志，2023，38（4）： 446-448.
[15] KROTULSKI A J, WALTON S E, FOGARTY M F, et al. NPS discovery toolkit: Metonitazene[EB/OL]. [2024-02-17]. https://www.cfsre.org/images/toolkits/Metonitazene_NPS-Discovery-Toolkit_072021.pdf.
[16] 唐泊伊，花镇东，王优美，等. 核磁共振扩散排序谱原位分离鉴定烟油中的美托尼秦[J/OL]. 分析试验室，2024： 1-10.[2024-02-17]. https：//kns.cnki.net/kcms/detail/11. 2017.tf.20241031.1621.026.html.

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电子烟油中尼秦类新精神活性物质美托尼秦的鉴定分析

Identification of Metonitazene, a Nitazene New Psychoactive Substance in E-liquids

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