主管：中华人民共和国司法部
主办：司法鉴定科学研究院
ISSN 1671-2072  CN 31-1863/N
中国司法鉴定

›› 2005 ›› Issue (01): 15-17.

• 论文 • 上一篇    下一篇

家兔玻璃体液HBDH、LDH活性变化与PMI相关性的研究

刘承泉,王伟平,龙仁,熊平   

  1. 湘潭市中级人民法院,衡阳市中级人民法院,南华大学法医病理教研室,南华大学法医病理教研室 湖南 湘潭 411100 ,湖南 衡阳 421001 ,湖南 衡阳 421001 ,湖南 衡阳 421001

Study on the Correlation Between PMI and Vitreous Humor of Rabbits

LIU Cheng-quan, WANG Wei-ping, LONG Ren, XIONG Ping(People's Court of Xiangtan, Xiang tan 411100, China;People's Court of Hengyang, Hen yang 421001, China;Department of Forensic Medicine, Nanhua University, Hen yang 421001,China)   

被引次数

6

摘要： 目的 寻找一种推断PMI的新方法。方法 应用全自动生化检测仪(用比色法)检测家兔死后0-54小时玻璃体液内羟丁酸脱氢酶(HBDH)、乳酸脱氢酶(LDH)在两组温度下(25-30℃:10-15℃)的失活情况。结果 两种酶的活性在死后一定时间内各出现一个平台期,平台期后两酶的活性迅速下降,低温组在死亡54小时后活性几乎为零,高温组在死亡48小时后活性几乎为零。经统计学分析得知死后两种酶活性与PMI之间有显著的负相关性(P<0.05)。结论 在0-54小时内依据其各自的回归方程将可以大致地推断PMI,依据两种酶活性的多元回归方程可以较准确地推断PMI。

关键词: 死后经过时间, 玻璃体液, 酶活性, HBDH, LDH, 家兔

Abstract: Objective To seek a new way of estimating postmortem interval (PMI). Methods Comparing obscurity was used to measure the two enzymes' activity of HBDH and LDH in vitreous humor of rabbits under different ambient temperatures (25-30℃;10-15℃) in the course of 0-54 hours after death. Results There was a different plateau period for the two enzymes' activity after a short period of death and the activity decreased quickly after the plateau period, reaching zero after 54 hours of death in low temperature groups and 48 hours in high ones. There existed high negative correlativity between the two enzymes' activity and PMI at a given range (P<0.05). Conclusion PMl can be roughly estimated by using simple regression equation and accurately estimated by using multiple regression equation.

Key words: postmortem interval (PMI), vitreous humor, enzyme 's activity, rabbit