主管：中华人民共和国司法部
主办：司法鉴定科学研究院
ISSN 1671-2072  CN 31-1863/N
中国司法鉴定

›› 2005 ›› Issue (04): 22-23+2.

• 论文 • 上一篇    下一篇

酶促试纸条测定唾液中乙醇含量的研究

丁国兴,王群,江勇,石琪,宋树强,张凯   

  1. 天津市刑事科学技术研究所,天津市刑事科学技术研究所,天津市刑事科学技术研究所,天津市刑事科学技术研究所,天津市刑事科学技术研究所,天津市刑事科学技术研究所 天津 300193 ,天津 300193 ,天津 300193 ,天津 300193 ,天津 300193 ,天津 300193

A Study on Test Paper of Alcohol in Saliva

DING Guo-xing, WANG Qun, JIANG Yong, SHI Qi, SONG Shu-qiang, ZHANG Kai (Tianjin Institute of Criminal Science Technology, Tianjin 300193, China)   

摘要： 生化酶促反应使试纸与含有乙醇的液体接触后显色,根据颜色的不同确定被测液体中的乙醇含量,依此研制出酶促试纸条。通过志愿者实验,同时收集血液和唾液, 比较酶促试纸条测定唾液中乙醇含量与顶空气相色谱检测血醇和唾醇的结果。考察酶促试纸条的影响因素。通过提高缓冲液浓度至0.6mol/L和减少乙醇氧化酶的量改进酶促试纸条,使相同乙醇浓度的酸碱两种唾液间的差异显著缩小。所研制的酶促试纸条具有成本低、操作简便、快速的优点。

关键词: 酶促试纸条, 唾液, 乙醇含量, 显色

Abstract: A kind of fast test paper for examining alcohol content in saliva was developed successfully by Tianjin Institute of Criminal Science and Technology. The paper contains a special enzyme which con react with alcohol in saliva and make the paper change colors in accordance with alcohol concentration. However, it was found that the color development was not fully consistent with the corresponding alcohol content in saliva for some people. Our study showed that the pH value in saliva was the main cause. Details of the problem were discussed.

Key words: pH value of saliva, examination of alcohol, color-fest paper