主管：中华人民共和国司法部
主办：司法鉴定科学研究院
ISSN 1671-2072  CN 31-1863/N
中国司法鉴定

›› 2007 ›› Issue (03): 19-22.

• 论文 • 上一篇    下一篇

外源性DHEA的体内代谢研究

沈敏;向平;沈保华;王萌烨;严慧;   

  1. 司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所 上海200063,上海200063,上海200063,上海200063复旦大学上海医学院法医系,上海200032,上海200063复旦大学上海医学院法医系,上海200032

Study on the Metabolism of Exogenous DHEA and Its Metabolites Level

SHEN Min1, XIANG Ping1, SHEN Bao-hua1, WANG Meng-ye1,2, YAN Hui1,2 (1. Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, China; 2. Forensic Medicine Department of Shanghai Medical College, Fudan University, Shanghai 20032, China)   

被引次数

3

摘要： 目的:考察中国健康成人尿液中DHEA及其代谢物水平,评价滥用外源性DHEA后尿液中DHEA的代谢状况及其对内源性蛋白同化激素水平的影响。方法:六名健康志愿者口服120mgDHEA进行代谢试验,依据已建立的LC-MS/MS分析方法,测定受试者尿液中DHEA、睾酮、表睾酮、雄酮和苯胆烷醇酮的水平。结果:受试者尿液中内源性睾酮浓度明显低于国内外运动员。单剂量服用DHEA后,尿液中DHEA、雄酮和苯胆烷醇酮的浓度有明显的升高,达峰时间分别为:3.9±0.6h、3.4±0.9h和3.6±0.9h,并且均在12h后恢复至原有正常浓度范围。结论:监测滥用外源性的DHEA时,其尿液中的DHEA浓度是有效的技术指标。

关键词: DHEA及其代谢物, 外源性, 内源性, 水平, 代谢

Abstract: Objective To observe the level of DHEA and its metabolites in urine from Chinese adults, and to evaluate the metabolism of DHEA in urine and its effects on the level of endogenous anabolic androgenic steroids. Methods Six healthy subjects' urine was collected at different time after administration of 120mg DHEA. The concentration of DHEA and its metabolites testosterone, epitestosteorne, androsterone and etiocholanolone in urine was measured by an improved LC-MS/MS method. Results The concentration of urinary testosterone was considerably lower in the volunteers than that in Chinese and foreign athletes. After oral administration of DHEA, DHEA and its major metabolites androsterone,etiocholanolone increased significantly in urine with peak levels at 3.9±0.6h, 3.4±0.9h and 3.6±0.9h, respectively, and then achieved normal after 12 hours. Conclusion The urine level of DHEA is an effective parameter in evaluating exogenous DHEA.

Key words: DHEA and its metabolites, exogenous, endogenous, level, metabolism