主管：中华人民共和国司法部
主办：司法鉴定科学研究院
ISSN 1671-2072  CN 31-1863/N
中国司法鉴定

›› 2010 ›› Issue (01): 26-29.

• 论文 • 上一篇    下一篇

保存犬尸体中自生醇的研究

刘晓霞;乔君元;贠克明;张大明;尉志文;安建康;王玉瑾;   

  1. 山西医科大学法医学院;太原市疾病预防控制中心理检科;太原市公安局杏花岭分局;北京市公安局法医检验鉴定中心;

Study on Self-generation Alcohol in Preserved Dog Corpses

LIU Xiao-xia1,4,QIAO Jun-yuan2,YUN Ke-ming1,ZHANG Da-ming3,WEI Zhi-wen1,AN jian-kang1,WANG Yu-jin1 (1. Forensic Medicine School of Shanxi Medical University,Taiyuan 030001,China; 2. Xinghualing Branch of Taiyuan Public Security Bureau,Taiyuan 030001,China   

摘要： 目的建立组织中自生醇直接进样毛细管柱气相色谱检测方法;建立犬尸体中自生醇研究模型,观察犬尸体中自生醇的产生情况。方法犬经CO2处死,置于室温,分别于死后0、2、4、8、12、24、48、72、96、120h采集样品,样品匀浆后(体液除外)离心去上清,内标溶液1∶2(体液为1∶4)稀释,直接进样,气相色谱法检测,保留时间定性,内标法定量。结果甲醇、丙酮、乙醇、正丙醇、异丁醇、异丙醇、正丁醇、异戊醇的保留时间分别为(2.77±0.01)、(3.42±0.01)、(3.87±0.01)、(5.92±0.01)、(7.54±0.01)、(7.92±0.01)、(10.33±0.01)、(13.55±0.01)min;大部分组织如心血、下腔静脉血、尿、胆汁、肝、脾、肺和肾在犬死后12h可检出乙醇,肌肉、玻璃体液、和大脑分别在死后48、72、96h检出乙醇;乙醇和正丙醇的生成存在正相关。结论死亡犬体内会产生自生醇,尿中检出乙醇不能判定为生前饮酒,在涉及乙醇的法医学检案中,特别是腐败样品,应注意自生醇对乙醇定性和定量可能造成的影响,应采集不易腐败的组织如玻璃体液、肌肉或大脑,检测乙醇及自生醇含量,并观察与正丙醇的比例关系来进行综合判定。

关键词: 乙醇, 自生醇, 气相色谱, 直接进样

Abstract: Objective To establish an analysis of alcohol and self-generation alcohol in biological samples by a direct-injection gas chromatogram; to develop an animal model for self-generation alcohol research and observe self-generation alcohol in pre-served dog corpses. Methods After executed by CO2,dogs were stored at room temperature and were sampled in 0,2,4,8,12,24,48,72,96,120h. The samples were homogenated and diluted (1:2 for tissue homogenate,1:4 for body fluid) with the inter-nal standard solution. Using a direct-injection gas chromatogram,the alcohol and self-generation alcohol in the samples were analyzed qualitatively and quantitatively by the retention time and internal standard method. Results The retention times of methanol,acetone,alcohol,n-propyl alcohol,isobutanol,isopropanol,n-butanol,isoamyl alcohol were (2.77±0.01)min,(3.42± 0.01)min,(3.87±0.01)min,(5.92±0.01)min,(7.54±0.01)min,(7.92±0.01)min,(10.33±0.01)min,(13.55±0.01)min,respec-tively. Alcohol was detected in heart-blood,inferior vena cava blood,urine,bile,liver,spleen,lung,kidney in 12h after death,and in muscle,humor vitreous,brain in 48,72,96h. There was a positive correlation between alcohol and n-propyl alcohol. Conclusion Alcohol can be produced postmortemly in dog corpses. Alcohol detected in urine can not be thought as the marker of antemortem drinking. In forensic identification of alcohol-related cases,self-generation alcohol should be taken into consid-eration. The unrotten tissues such as muscle,humor vitreous and brain should be sampled for the determination of alcohol and self-generation alcohol,and the ratio of alcohol and n-propyl alcohol in samples could be used for identification of antemortem drinking.

Key words: alcohol, self-generation alcohol, gas chromatogram, direct-injection