主管：中华人民共和国司法部
主办：司法鉴定科学研究院
ISSN 1671-2072  CN 31-1863/N
中国司法鉴定

›› 2013 ›› Issue (3): 0-0.

• 鉴定科学 •    下一篇

人体脂肪的高效液相色谱-蒸发光散射分析

王磊,史晓凡,许英健,张芳   

  1. 中国刑事警察学院
  • 收稿日期:2012-07-27 修回日期:2013-05-03 出版日期:2013-05-15 发布日期:2013-06-04
  • 通讯作者: 史晓凡
  • 基金资助:

    公安部重点项目计划项目

Analysis of Human Fat by HPLC-ELSD

  • Received:2012-07-27 Revised:2013-05-03 Published:2013-05-15 Online:2013-06-04

被引次数

4

摘要：  目的建立凶杀案件现场遗留的微量人体脂肪残渣种属的检验鉴定方法。方法用丙酮溶解人体油脂、动物油脂，油脂溶液进行高效液相色谱-蒸发光散射分析。结果人体脂肪的液相色谱数据特征性强，与常见动物油脂差异显著，可作为鉴定人体脂肪种属的依据。检材不受各种环境因素的干扰，对于腐败的人体脂肪仍可得出准确鉴定结论。结论高效液相色谱-蒸发光散射方法是检验鉴定微量人体脂肪种属的一种有效手段。

Abstract: Objective To establish a method for identification of trace amount human fat residues at crime scenes. Method Human and animal fats are dissolved in acetone, and analyzed by high performance liquid chromatography with evaporative light-scatter detector (HPLC-ELSD). Results The chromatogram of human fat is obviously different from that of animal fats, which could be used to identify the fat of human source. Samples are not interfered by environmental factors. Even with decaying human fat, correct analysis results could still be got. Conclusion The established HPLC-ELSD method is effective in identifying trace amount human fat.