Abstract: Objective To investigate the characteristic differences between the impressions of homologous laser-carving osmotic stamps in order to establish a method for the identification of this kind of stamp impressions. Method 9 homologous laser-carving osmotic stamps were manufactured and multiple impressions were imprinted. The characteristics of the impressions were analyzed with microscopes, Video Spectral Comparator, and other instruments. Results The detailed characteristic differences of homologous laser-carving osmotic stamp impressions were found out. Conclusion Although the specifications of homologous laser-carving osmotic stamp impressions agree well, they can still be discriminated. The identification should focuses on the comparison of detailed characteristics.