主管：中华人民共和国司法部
主办：司法鉴定科学研究院
ISSN 1671-2072  CN 31-1863/N
中国司法鉴定

›› 2013 ›› Issue (5): 48-50.

• 鉴定实践 • 上一篇    下一篇

植物DNA条行码技术用于一起交通命案的调查

雷湘华1,高必达2,周倩1   

  1. 1. 湖南农业大学生物安全科学技术学院
    2. 湖南农业大学司法鉴定中心
  • 收稿日期:2013-03-27 修回日期:2013-05-14 出版日期:2013-09-15 发布日期:2013-10-14
  • 通讯作者: 高必达

The application of plant gene barcode technology in a traffic forensic case

  • Received:2013-03-27 Revised:2013-05-14 Published:2013-09-15 Online:2013-10-14

摘要： 目的 以rDNA-ITS序列片段为植物DNA条形码对涉及一例交通命案的植物检材进行种属鉴定，判断三轮车左侧车体上提取的树皮样物质与农用车所运的树木是否为同一树种。方法 采用改良CTAB法提取植物DNA，先将DNA纯化，再进行rDNA-ITS的PCR扩增，然后对PCR产物切胶、回收，克隆、测序，测序结果进行BLAST分析，并构建系统发育树。结果 三轮车上粘附的树皮来源于柑橘(Citrus sunki)，农用车所运的树木为界山三角槭(Acer buergerianum)，两者非同一种属。结论 用rDNA-ITS序列片段作为植物DNA条形码对植物种属进行鉴定，可获得可靠的鉴定意见。

关键词: 物种鉴定, rDNA-ITS序列, 植物条行码基因, 交通命案, species identification, rDNA-ITS sequence, plant barcode gene, traffic murder

Abstract: Objective To identify the wood species of the detected plant materials involving in a traffic case.The rDNA-ITS sequence fragments of plant DNA barcode were analyzed for determining whether the bark-like materials collected from the left side of the tricycle were the same species as those carried on the agricultural vehicles. Method The plant DNA was extracted by the modified CTAB method. rDNA-ITS PCR amplification was performed and then the PCR products were cloned, sequenced, and the information of sequences was used for Blast analysis and phylogenetic tree construction. Result The adhesional substances on tricycle were originated from the Citrus sunki, while the plant shipped in the agricultural vehicles was Acer buergerianum (the variant species of JieShan Triangle), which was inconsistent with that of the bark on tricycle. Conclusion rDNA-ITS sequence fragments as plant DNA barcode could be applied for plant species identification.