主管：中华人民共和国司法部
主办：司法鉴定科学研究院
ISSN 1671-2072  CN 31-1863/N
中国司法鉴定

›› 2014 ›› Issue (1): 56-58.

• 鉴定实践 • 上一篇    下一篇

采用Pyrosequencing和Pooling技术对SNP位点多态性分析

赵珍敏   

  1. 司法部司法鉴定科学技术研究所
  • 收稿日期:2013-12-03 修回日期:2013-09-29 出版日期:2014-01-15 发布日期:2014-02-13
  • 通讯作者: 赵珍敏

Polymorphism Analysis of SNP Loci by Pyrosequencing and Pooling

  • Received:2013-12-03 Revised:2013-09-29 Published:2014-01-15 Online:2014-02-13

摘要： 目的 建立基于pyrosequencing和Pooling技术进行SNP位点的法医学多态性分析技术。方法 对50名无关个体样本建立一适合pyrosequencing检测的组池；采用PyroMark Assay Design 2.0软件进行SNP位点等位基因定量分析的引物设计；对组池样本PCR产物进行焦磷酸测序检测。结果 检测的3个SNP位点多态性良好，其中位点rs220028与以往人群调查后频率数据无显著差异。结论 采用pyrosequencing和Pooling技术对SNP位点进行多态性分析，适合于位点的初筛及大规模群体调查。该技术准确可靠，方便快捷。

关键词: pyrosequencing, Pooling, SNP

Abstract: Objective To establish a method for SNP polymorphism analysis based on pyrosequencing and pooling technology. Methods A sample pooling of 50 unrelated individuals was set up for pyrosequencing testing. PyroMark Assay Design 2.0 software was adopted to design primer for allelic quantification. Afterwards, PCR products of the pooling samples were pyrosequenced. Results The polymorphism of three target SNP loci was observed. Moreover, for rs220028, there is no significant difference between the testing results and previous data based on population survey. Conclusion The established SNP analysis method based on pyrosequencing and pooling technology is accurate, reliable and easy to perform. It is suitable for the screening and investigation of large population.

Key words:  pyrosequencing, pooling, SNP