主管:中华人民共和国司法部
主办:司法鉴定科学研究院
ISSN 1671-2072  CN 31-1863/N

中国司法鉴定 ›› 2010 ›› Issue (5): 38-42.

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死后大鼠脾脏组织FTIR测量结果的法医学分析

黄 平1,2,余荣军3,李 立1,4   

  1. 1. 司法部司法鉴定科学技术研究所 上海市法医学重点实验室,上海 200063; 

    2. 西安交通大学 医学院 法医学系,陕西 西安710061; 3. 宁波市公安局 刑侦支队,浙江 宁波 315040; 

    4. 复旦大学 上海医学院 法医学系,上海 200032 

  • 收稿日期:2010-04-02 出版日期:2010-09-15 发布日期:2022-07-06
  • 通讯作者: 王振原(1964-),男,教授,博士研究生导师。
  • 作者简介:黄平(1979- ),男,博士,助理研究员,主检法医师,主要从事法医病理学研究。E-mail:huangpingxjtu@gmail.com
  • 基金资助:

    国家自然科学基金资助项目(30471935);上海市自然科学基金资助项目(09ZR1432900)

Forensic Medical Analysis of FTIR Spectral Changes of Rat Spleen Postmortem

HUANG Ping1,2, YU Rong-jun3, LI Li1,4   

  1. 1. Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of justice, Shanghai 200063, China; 

    2. Department of Forensic science, School of Medicine, Xi’an Jiaotong University, Xi’an 710061, China;

    3. Ningbo Public Security Bureau, Ningbo 315040, China; 

    4. Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China 

  • Received:2010-04-02 Published:2010-09-15 Online:2022-07-06

摘要: 目的 应用傅里叶变换红外( FTIR) 光谱技术分析大鼠死后脾脏组织随死亡时间增加的化学变化过程,为死亡时间推断研究提供新的途径与研究数据。方法 大鼠断颈处死后,在30℃、20℃及4℃环境中,不同死亡时间点提取大鼠脾脏组织,并运用FTIR光谱仪测定不同化学基团随死亡时间的变化。结果 随着死亡时间的推移,大鼠脾脏组织FTIR光谱的主要吸收峰峰位没有明显变化,而其吸收峰强度有明显差异:(1)1 080 cm-1和1 238 cm-1被指认核酸谱带吸收峰的峰强呈下降趋势;(2)1 541 cm-1被指认酰胺Ⅱ吸收峰的峰强呈上升变化;(3)1 396cm-1被指认脂肪酸吸收峰的峰强呈上升变化;(4) 指认为C-H结构振动的2 852、2 871、2 923、2 958cm-1吸收峰的峰强呈现上升趋势。结论 FTIR光谱分析技术有望成为法医死亡时间推断的有效方法。

关键词:

font-family:", font-size:10.5pt, "> 傅立叶变换;红外光谱;死亡时间;脾脏

Abstract: Objective To explore chemical changes in the rat spleen postmortem using FTIR spectroscopy and to provide a new method and research data for estimation of postmortem interval. Methods Rats were sacrificed by cervical dislocation and the cadavers were kept at 30℃, 22℃-26℃ and 4℃ in a controlled environment chamber. The spleens were sub-samples at different postmortem time points. All spleen tissues were measured using FTIR spectrometer. Results The absorption peak position showed no changes after death, but absorption intensity displayed obvious changes: (1)the absorption intensity assigned to nucleic acid (1080 cm-1 and 1238 cm-1) showed decreasing tendency; (2)the absorption intensity assigned to amide Ⅱ(1541 cm-1) showed increasing tendency; (3)the absorption intensity assigned to fatty acid (1396 cm-1) increased. (4)the absorption intensity assigned to C-H vibration (2852, 2871, 2923, 2958cm-1) showed increasing tendency. Conclusion FTIR spectroscopy can be an effective method for the estimation of postmortem interval.

Key words:

font-family:", font-size:10.5pt, "> Fourier transform; infrared spectrometry; postmortem interval; spleen

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