主管：中华人民共和国司法部
主办：司法鉴定科学研究院
ISSN 1671-2072  CN 31-1863/N
中国司法鉴定

›› 2007 ›› Issue (03): 28-30.

• 论文 • 上一篇    下一篇

实时DNA定量技术的应用研究

李成涛;林源;柳燕;李莉;   

  1. 司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所 上海200063,上海200063,上海200063,上海200063

Measurement of DNA Copy in Forensic Samples Using Real Time Quantitative PCR Technique

LI Cheng-tao, LIN Yuan, LIU Yan, LI Li (Institute of Forensic Sciences, Ministry of Justice, Shanghai 200063, China)   

被引次数

25

摘要： 通过应用TaqMan探针法和SYBR Green荧光染料法对常见的法医生物学检材进行DNA定量,对实时荧光定量PCR技术用于法庭科学样品定量检测的适用性进行研究。实验结果表明,该技术在DNA定量及抑制因素评估等方面具有重要的法医学应用价值。

关键词: 荧光定量PCR技术, 定量, 法医物证学

Abstract: The DNA copy of samples was detected by applying a real-time quantitative PCR assay with TaqMan Probe and SYBR Green. DNA was abstracted from variety of biological samples common in forensic science. Experiment results show that the real time quantitative PCR has great forensic application potential in the DNA copy quantification and evaluation of inhibiting factors of abstracted DNA.

Key words: fluorescent quantitative PCR, quantification, forensic biological evidence