Distribution of Five Sedative Hypnotics and Metformin in Hair by LC-MS/MS

doi:10.3969/j.issn.1671-2072.2025.01.003

Chinese Journal of Forensic Sciences ›› 2025 ›› Issue (1): 19-27.DOI: 10.3969/j.issn.1671-2072.2025.01.003

• Special Topic Research: Personnel Profiling Technologies Based on Physical and Chemical Analysis • Previous Articles Next Articles

Distribution of Five Sedative Hypnotics and Metformin in Hair by LC-MS/MS

ZOU Bo, CHANG Jing, WANG Aihua, WU Xiaojun, LI Jiayi, ZHANG Yunfeng

Beijing Municipal Engineering Technology Research Center for Crime Scene Evidence Examination, Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China

Received:2024-09-10 Published:2025-01-15 Online:2025-01-15

毛发中5种镇静催眠药物和二甲双胍的LC-MS/MS检验及分布特征研究

邹波，常靖，王爱华，吴小军，李佳宜，张云峰

公安部鉴定中心北京市现场物证检验工程技术研究中心，北京 100038

通讯作者: 张云峰（1983—），女，警务技术正高级任职资格，硕士，主要从事法医毒物检验。E-mail：zhangyunfeng2009@foxmail.com
作者简介:邹波（1988—），男，警务技术副高级任职资格，博士，主要从事法医毒物检验。E-mail：zoubo1141@126.com
基金资助:
“十四五”国家重点研发计划课题（2023YFC3303903）

Abstract

Abstract: Objective To study the distribution of five hypnotics (diazepam, zolpidem, et al) and metformin in the hair samples of users, providing reference for characterizing the medication history. Methods The hair samples were segmented along the growth direction, ground and extracted. The content in each hair segment was determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and analyzed with the donors’ medication information. Results The distribution range of diazepam, alprazolam, clonazepam, and metformin in the hair samples corresponded to the duration of medication. The contents of analytes were related to multiple factors such as physical and chemical properties of the drugs, dosage, hair color, and individual metabolic differences. Conclusion The segmental hair analysis and drug distribution characteristics of real users can provide basic data support for the characterization of donors’ medication history in case investigation.

Key words: hair sample, segmental analysis, medication history profile, sedative hypnotics, metformin

摘要： 目的研究地西泮、唑吡坦等镇静催眠药物及二甲双胍在用药人员毛发中的含量分布特征，为毛发供体的摄药史刻画提供参考。方法采集真实用药人员毛发，沿生长方向分段后研磨提取，采用液相色谱-串联质谱法（liquid chromatography-tandem mass spectrometry，LC-MS/MS）检测各段药物含量，并与供体用药信息进行关联分析。结果地西泮、阿普唑仑、氯硝西泮、二甲双胍在用药人员毛发中的分布区间与用药时间存在良好对应关系，药物含量与药物理化特性、用药剂量、头发颜色及个体代谢等多重因素相关。结论真实用药人员的头发分段检验和药物分布特征分析，能够为涉案毛发样本的供体摄药史刻画提供基础数据支撑。

关键词: 头发, 分段检验, 摄药史刻画, 镇静催眠药物, 二甲双胍

CLC Number:

DF795.1

ZOU Bo, CHANG Jing, WANG Aihua, WU Xiaojun, LI Jiayi, ZHANG Yunfeng. Distribution of Five Sedative Hypnotics and Metformin in Hair by LC-MS/MS[J]. Chinese Journal of Forensic Sciences, 2025(1): 19-27.

邹波, 常靖, 王爱华, 吴小军, 李佳宜, 张云峰. 毛发中5种镇静催眠药物和二甲双胍的LC-MS/MS检验及分布特征研究[J]. 中国司法鉴定, 2025(1): 19-27.

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Distribution of Five Sedative Hypnotics and Metformin in Hair by LC-MS/MS

毛发中5种镇静催眠药物和二甲双胍的LC-MS/MS检验及分布特征研究

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