Objective To discuss the methods of identifying the action manners of victims in road traffic accidents. Method 28 cases of the identification of victims * action manners in road traffic accidents were collected. The general status of the individual, the degree of injury, the type of collision in road traffic accident, and the forensic techniques which were adopted in these cases were recorded and analyzed statistically. Results There were 57 victims involved in 28 cases of road traffic accidents, including 27 dead, 29 injured, and 1 uninjuried. The degrees of injury were as follow: 10 cases of slight injury (34.5%), 8 cases of minor injury (27.6%), and 11 cases of severe injury (37.9%). As to the type of collision, 11 cases were between automobiles (39.3%), 5 cases were between electric-bicycle and automobile (17.8%), 1 case was between car and bicycle (3.6 %), 7 cases were between automobile and immovable object (25.0%), and 4 cases were between automobile and pedestrian (14.3%). The action manners of victims identified were as follows: the driver and the passengers (16 cases, 57.1%), the motorcyclist and the passenger (1 case, 3.6%), the electric-bicyclist and the passenger (5 cases, 17.8%), the bicyclist pushing and bicyclist riding (1case, 3.6%), the motorcyclist pushing and motorcyclist riding (1 case, 3.6%), pedestrian crossing road (4 cases, 4.3%). The techniques adopted were forensic clinical examination, forensic corpse surface examination, vehicle trace examination, forensic DNA analysis, and simulation experiments. Conclusion The basic approach of identifying action manners of victims in road traffic accidents is to analyze the injury objects and the injury manner, according to the morphological characteristics of injuries, the result of vehicle trace examination and the information of the specific road traffic accident. Then, the process of a road traffic accident could be reconstructed. As a result, the judgment whether or not the action manner of the victim is accorded with a specific manner of action could be obtained.