主管：中华人民共和国司法部
主办：司法鉴定科学研究院
ISSN 1671-2072  CN 31-1863/N
中国司法鉴定

中国司法鉴定 ›› 2025 ›› Issue (2): 40-46.DOI: 10.3969/j.issn.1671-2072.2025.02.005

• 鉴定科学 • 上一篇    下一篇

超高效液相色谱-串联质谱法同时测定血液中8种苯环利定类物质

杨  洲1，石恺婷2，杨荣极1，李国娟1，杨 柳1，廖彩云1，徐布一1   

  1. 1. 国家毒品实验室四川分中心，四川 成都 610200； 2. 四川大学 华西基础医学与法医学院，四川 成都 610041
  • 收稿日期:2024-02-20 出版日期:2025-03-15 发布日期:2025-03-25
  • 作者简介:杨洲（1996—），男，工程师，主要从事毒物毒品分析研究。E-mail：1194517431@qq.com
  • 基金资助:
    四川省科技计划项目（2023YFS0418）；毒品监测管控与禁毒关键技术公安部重点实验室开放课题（2022-KLDMC-07）

Simultaneous Analysis of 8 Phencyclidine-type Substances in Blood by UHPLC-MS/MS

YANG Zhou1, SHI Kaiting2, YANG Rongji1, LI Guojuan1, YANG Liu1, LIAO Caiyun1, XU Buyi1   

  1. 1. National Anti-Drug Laboratory Sichuan Regional Center, Chengdu 610000, China; 2. West China School of Basic Medicine Sciences & Forensic Medicine, Sichuan University,Chengdu 610041, China
  • Received:2024-02-20 Published:2025-03-15 Online:2025-03-25

PDF

摘要： 目的 建立同时测定血液中8种苯环利定（phencyclidine，PCP）类物质的超高效液相色谱-串联质谱检测方法。方法 使用乙腈蛋白沉淀法处理血液，采用ACQUITY UPLC BEH C18 色谱柱（2.1 mm×100 mm，1.7 μm），以0.1%（体积分数）甲酸溶液-含0.1%甲酸溶液的乙腈为流动相进行梯度洗脱，流速 0.4 mL/min。质谱检测采用电喷雾离子源正离子、动态多反应监测模式。结果 该方法实现了对血液中8种苯环利定类物质的定性、定量分析，各目标物在0.1~50 ng/mL范围内具有良好的线性关系，相关系数均大于0.997，检出限为0.015~0.03 ng/mL，定量限为0.05~0.1 ng/mL。该方法成功应用于实际血液，于该血液中检出2-FXE成分。结论 该方法具有快速、灵敏、准确、适用性广等突出特点，能够满足血液中苯环利定类物质的定性、定量分析要求，并为分析未来新出现的苯环利定类物质提供参考。

关键词: 苯环利定类物质, 血液, 乙腈蛋白沉淀法, 超高效液相色谱-串联质谱法

Abstract: Objective To establish a determination method of ultra-high performance liquid chromatography-tandem mass spectrometry （UHPLC-MS/MS） for simultaneous analysis of 8 phencyclidine-type substances in blood. Methods Processing blood using acetonitrile protein precipitation method, use an ACQUITY UPLC BEH C18 column （2.1 mm×100 mm, 1.7 μm）, and select acetonitrile-water containing 0.1% （volume fraction） formic acid as the mobile phase at 0.4 mL/min. Mass spectrometry was detected using an electrospray ionization-positive ion, dynamic multiple reaction monitoring （MRM） detection mode. Results This method achieved qualitative and quantitative analysis of 8 phencyclidine-type substances in blood. Each target object has a good linear relationship in the range of 0.1-50 ng/mL, with the correlation coefficients all greater than 0.997. The minimum detection limits range from 0.015 ng/mL to 0.03 ng/mL, and the minimum quantitative limits ranged from 0.05 ng/mL to 0.1 ng/mL. The proposed method was successfully applied to actual blood and detected the 2-FXE component in the blood. Conclusion This method is rapid, sensitive, accurate and has wide applicability, which can meet the requirements of qualitative and quantitative analysis of phencyclidine-type substances in blood. It can also provide a reference for analyzing new phencyclidine-type substances in the future.

Key words: phencyclidine-type substance, blood, acetonitrile protein precipitation method, UHPLC-MS/MS

中图分类号: