主管：中华人民共和国司法部
主办：司法鉴定科学研究院
ISSN 1671-2072  CN 31-1863/N
中国司法鉴定

›› 2008 ›› Issue (01): 40-41+4.

• 论文 • 上一篇    下一篇

Identifiler和Sinofiler试剂盒在亲权鉴定中的应用与评估

林源;郭宏;赵珍敏;李莉;   

  1. 司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所,司法部司法鉴定科学技术研究所 上海200063,上海200063 苏州大学基础医学院,苏州215123,上海200063,上海200063

Application and Evalution of Identifiler and Sinofiler Kit in Forensic Paternity Testing

LIN Yuan1, GUO Hong1,2, ZHAO Zhen-ming1, LI Li1(1. Institute of Forensic Science, Ministry of Justice, Shanghai 200063, China; 2.Medical School of Soochow University, Suzhou 215123, China)   

被引次数

11

摘要： 目的应用与评估Identifiler和Sinofiler试剂盒在亲权鉴定中的应用价值。方法应用五色荧光标记复合扩增系统对106例亲权鉴定的血样进行PCR扩增,用ABI3100-Avant遗传分析仪对扩增产物进行毛细管电泳,用GeneMapper v3.2软件进行基因分型,评估Identifiler和Sinofiler试剂盒在中国汉族人群中的适用性。结果在不排除的案例中Sinofiler比Identifiler具有较高的PI值,在排除的案例中Sinofiler比Identifiler具有较多的排除指标。结论Sinofiler比Identifiler更适用于中国汉族人群的亲权鉴定。

关键词: Identifiler试剂盒, Sinofiler试剂盒, TH01, TPOX, D6S1043, D12S391, 亲权鉴定, 短串联重复序列

Abstract: objective To apply and evaluate two different kits-- Identifiler and Sinofiler in paternity testing of Chinese Han population. Methods By multiplex amplification system, capillary electrophoresis and GeneMapper typing, 15 STR loci were genotyped in 106 cases of paternity testing. Results In inclusive cases, Sinofiler has higher PI valves than Identifiler; in exclusive cases, Sinofiler is more effective than Identifiler. Conclusion The results show that Sinofiler is more suitable in Chinese Han paternity testing.

Key words: Identifiler kit, Sinofiler kit, TH01, TPOX, D6S1043, D12S391, paternity testing, STR