主管：中华人民共和国司法部
主办：司法鉴定科学研究院
ISSN 1671-2072  CN 31-1863/N
中国司法鉴定

中国司法鉴定 ›› 2012 ›› Issue (1): 20-41.

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高效液相色谱法鉴别染料型黑色喷墨打印字迹

尹宝华1，2，郭瑞华3，梁鲁宁2等   

  1. 1.中国人民公安大学，北京 100038； 2.公安部物证鉴定中心，北京 100038； 3.首都师范大学，北京 100048
  • 收稿日期:2011-06-30 出版日期:2012-01-15 发布日期:2022-07-12
  • 作者简介:尹宝华（1977-），女，硕士，助理研究员，主要从事文件材料检验鉴定.
  • 基金资助:
    中央级公益性科技院所专项资金项目（2009JB014）

Discrimination of Dye-Based Black Inkjet Entries by HPLC

YIN Bao-hua1，2， GUO Rui-hua3， LIANG Lu-ning2，etc.   

  1. 1.Chinese People’s Public Security University， Beijing 100038， China； 2. Institute of Forensic Science， Ministry of Public Security， Beijing 100038， China； 3. Capital Normal University， Beijing 100048， China
  • Received:2011-06-30 Published:2012-01-15 Online:2022-07-12

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被引次数

1

摘要：

目的 建立液相色谱分析方法，对市售的30 种不同厂家牌号（原装）染料型黑色喷墨打印字迹进行区分鉴别。方法 通过选择最佳的提取条件、流动相，考察纸张、字迹形成方式等的影响，及样品及仪器的稳定性实验，确定实验条件。结果 选择了最佳的反相高效液相色谱（RP-HPLC）分析方法：固定相为X-bridge Shield RP18（3.5 μm， 4.6×100 mm），流动相为乙腈-NH4HCO3溶液（10 mmol/L），梯度洗脱，检测波长为585 nm、 475 nm；用该方法，根据字迹染料成分的差异将30种样品分为四大类17小类。结论 该方法可以为鉴别区分不同厂家染料型黑色喷墨打印字迹提供依据。

关键词: 染料型, 喷墨打印墨水, 高效液相色谱法（HPLC）

Abstract:

Objective To establish an HPLC method for distinguishing printing entries made by thirty dye-based black inkjet of different trademarks and manufacturers. Methods In the experiment， the extraction solvents and mobile phase were optimized. The influence of the paper and the ways of printing were also investigated. In addition， the precision and repeatability of the established method were examined. Results The separation was performed on an X-bridge Shield RP18（3.5 μm，4.6×100 mm）column. Gradient elution was carried out with acetonitrile and ammonium bicarbonate solution （NH4HCO3， 10mmol/L） as the mobile phase. The detection wavelengths were 585 nm and 475 nm. Under this condition， according to the differences of the main dye components， the thirty dye-based black inkjet entries could be categorized into four main classes. Conclusion The study provides an effective， reliable and stable method for discrimination of different dye-based black inkjet entries.

Key words: dye-based, inkjet entry, high performance liquid chromatography（HPLC）

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