基于表面增强拉曼光谱的五种农兽药快速检测

doi:10.3969/j.issn.1671-2072.2025.03.008

中国司法鉴定 ›› 2025 ›› Issue (3): 59-66.DOI: 10.3969/j.issn.1671-2072.2025.03.008

基于表面增强拉曼光谱的五种农兽药快速检测

李亚玲^1，2，赵越杰³，胡向阳^1，2，周吉³

1. 中南财经政法大学刑事司法学院，湖北武汉 430073； 2. 司法鉴定技术应用与社会治理学科创新基地，
湖北武汉 430073； 3. 湖北大学化学化工学院，湖北武汉 430062

收稿日期:2024-07-30 出版日期:2025-05-15 发布日期:2025-05-16
通讯作者: 胡向阳（1964—），男，教授，博士，主要从事侦查学和物证技术学研究。E-mail：709667234@qq.com 周吉（1982—），女，副教授，博士，主要从事光谱学分析与应用。E-mail：zhouji@hubu.edu.cn
作者简介:李亚玲（1992—），女，讲师，博士，主要从事侦查学和物证技术学研究。E-mail：18602702126@163.com
基金资助:
2023年度国家社会科学基金一般项目（23BFX139）；教育部科技部司法鉴定技术应用与社会治理学科创新引智基地项目（B20077）

Rapid Detection of Five Agricultural and Veterinary Drugs by Surface-Enhanced Raman Spectroscopy

LI Yaling^{1, 2}, ZHAO Yuejie³, HU Xiangyang^{1, 2}, ZHOU Ji³

1. College of Criminal Justice, Zhongnan University of Economics and Law, Wuhan 430073, China;
2. Innovation Base of Forensic Technology Application and Social Governance, Wuhan 430073, China;
3. College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China

Received:2024-07-30 Published:2025-05-15 Online:2025-05-16

摘要/Abstract

摘要： 目的建立一种表面增强拉曼光谱（surface-enhanced Raman spectroscopy，SERS）分析方法用于结晶紫、孔雀石绿、亚甲基蓝三种兽药和福美双、噻菌灵两种农药的快速检测。方法以聚氨酯（polyurethane，PU）纱线为柔性衬底，在其表面修饰聚多巴胺（polydopamine，PDA）功能层，将修饰后的纱线（PU@PDA）浸入银氨溶液中，利用PDA的还原性将纱线表面的银氨络离子原位还原得到银纳米粒子（silver nanoparticles，AgNPs），即制备出PU@PDA@AgNPs复合柔性SERS基底材料。通过改变银氨溶液的浓度以调控PU@PDA上的AgNPs，优化复合功能材料的SERS传感性能，进而用于三种染料类兽药和两种农药的SERS检测。结果对结晶紫、孔雀石绿和亚甲基蓝的检测浓度均可低至1.0×10-8 mol/L，对农药福美双和噻菌灵的检测浓度分别低至0.5 mg/kg和2 mg/kg。结论该方法操作简便，环保安全，灵敏度高，可为农兽药残留的现场快速检测提供有力的技术支撑。

关键词: 表面增强拉曼光谱, 聚氨酯, 银纳米粒子, 农兽药

Abstract: Objective To establish a surface-enhanced Raman spectroscopy (SERS) method for the rapid detection of three forbidden veterinary drugs, crystal violet, malachite green, and methylene blue, as well as two pesticides, thiram and thiabendazole. Methods Polyurethane (PU) yarn was used as a flexible substrate, and its surface was further modified with polydopamine (PDA) functional layer. The modified yarn (PU@PDA) was then immersed in a silver ammonia solution. The silver ions in silver ammonia complex can be in-situ reduced to silver nanoparticles (AgNPs) by the reducibility of PDA. This produced the PU@PDA@AgNPs composite flexible SERS substrate material. Moreover, the sensitivity of the SERS material can be optimized by changing the concentration of silver ammonia solution to control the amount of AgNPs on PU@PDA yarn. The new material was then applied in the detection of three dye based forbidden veterinary drugs and two pesticides. Results The limits of detection for crystal violet, malachite green, and methylene blue can be as low as 1.0×10-8 mol/L, and the limits of detection for pesticides thiram and thiabendazole were as low as 0.5 mg/kg and 2 mg/kg, respectively. Conclusion This method is easy to operate, environmentally friendly, safe and highly sensitive. It can provide technical support for the rapid on-site detection of agricultural and veterinary drug residues.

Key words: surface-enhanced Raman spectroscopy (SERS), polyurethane (PU), silver nanoparticles (AgNPs), agricultural and veterinary drug

中图分类号:

DF793.3

李亚玲, 赵越杰, 胡向阳, 周吉. 基于表面增强拉曼光谱的五种农兽药快速检测[J]. 中国司法鉴定, 2025(3): 59-66.

LI Yaling, ZHAO Yuejie, HU Xiangyang, ZHOU Ji. Rapid Detection of Five Agricultural and Veterinary Drugs by Surface-Enhanced Raman Spectroscopy[J]. Chinese Journal of Forensic Sciences, 2025(3): 59-66.

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基于表面增强拉曼光谱的五种农兽药快速检测

Rapid Detection of Five Agricultural and Veterinary Drugs by Surface-Enhanced Raman Spectroscopy

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