主管：中华人民共和国司法部
主办：司法鉴定科学研究院
ISSN 1671-2072  CN 31-1863/N
中国司法鉴定

中国司法鉴定 ›› 2025 ›› Issue (5): 39-43.DOI: 10.3969/j.issn.1671-2072.2025.05.005

• 鉴定科学 • 上一篇    下一篇

亲子鉴定中母亲嵌合体STR分型研究

汪弯弯1，刘慧欣2，马静红1，王泊文2，吴玉剑3
  

  1. 1. 湖北崇新司法鉴定中心，湖北 武汉430400； 2. 许昌莲城司法鉴定所，河南 许昌461000； 3.武汉市公安司法鉴定中心，湖北 武汉430024
  • 收稿日期:2024-05-28 出版日期:2025-09-15 发布日期:2025-09-25
  • 通讯作者: 吴玉剑（1985—），男，警务技术正高级任职资格，博士,主要从事法医物证学科研及鉴定。E-mail：scmirror@163.com
  • 作者简介:汪弯弯（1991—），女，主要从事法医物证鉴定。E-mail：1299387832@qq.com

STR Genotyping Analysis of Maternal Chimerism in a Parentage Testing Case

WANG Wanwan1, LIU Huixin2, MA Jinghong1, WANG Bowen2, WU Yujian3   

  1. 1. Hubei Chongxin Forensic Appraisal Center, Wuhan 430400, China; 2. Xuchang Liancheng Forensic Appraisal Institute, Xuchang 461000, China; 3. Judicial Forensic Center of Wuhan Public Security Bureau, Wuhan 430024, China
  • Received:2024-05-28 Published:2025-09-15 Online:2025-09-25

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摘要： 目的 对1例三联体亲子鉴定中生母和女儿血样STR分型不符合遗传规律的原因进行分析。方法 利用GoldeneyeTM 20A试剂盒对被检父、女儿（小女儿）和生母血样进行基因分型检测及亲子鉴定，并进一步采集生母带毛囊的头发和口腔拭子及大女儿血样进行基因分型检测。结果 被检父与两个女儿均符合亲子关系，生母血样基因分型与小女儿基因分型排除亲子关系，与大女儿基因分型有2个基因座不符合遗传规律。生母头发中检出的基因分型与血样不同，且头发基因分型与两个女儿的基因分型均符合亲子关系。生母口腔拭子检出混合基因分型，包含其血样和头发的基因分型。 结论 本案例中母亲为罕见的嵌合体基因型，是两个女儿的生物学母亲。

关键词: 法医物证学, 亲子鉴定, 短串联重复序列, 嵌合体

Abstract: Objective To investigate the cause of the exclusion of a parent-child relationship between the biological mother and her daughters in a trio parentage test. Methods Genotyping and parentage testing were performed using the GoldeneyeTM 20A kit on blood samples from the alleged father, the daughter (younger daughter), and the biological mother. Additional samples, including the mother’s hair with follicles, buccal swab, and a blood sample from the elder daughter, were subsequently collected for further genotyping. Results The alleged father was confirmed to as the biological father of both daughters. However, the genotype obtained from the mother’s blood sample excluded her as the biological mother of the younger daughter and exhibited inconsistencies at two STR loci with the elder daughter’s genotype, violating Mendelian inheritance. A different genotype was detected in the mother’s hair sample, and the hair-derived genotype was consistent with her being the biological mother of both daughters. The mother’s buccal swab displayed a mixted genotype, comprising both the genetic profiles found in her blood and hair samples. Conclusion In this case, the mother was identified as a rare chimeric individual and is the biological mother of both daughters.

Key words: forensic genetics, parentage testing, short tandem repeats (STR), chimerism

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