关键词: N-乙酰邻氨基苯甲酸, 邻氨基苯甲酸, 苯乙酸, 微波衍生化, GC-MS, N-acetylanthranillic acid, anthranilic acid, phenylacetic acid, microwave derivatization, GC-MS

Abstract: Objective To establish a method for the identification of acid precursor chemicals by gas chromatography-mass spectrometry （GC-MS） with derivatization. Method Analysis of N-acetylanthranillic acid， anthranilic acid and phenylacetic acid was carried out by GC/MS combined with derivatization， with benzoic acid as the internal standard. Results The detection effect after microwave derivatization with pentafluorobenzylbromide （PFBBr） was better than that of direct detection， as the chromatographic peak and the sensitivity were significantly improved， and the limit of detection （LOD） was decreased. The characteristic fragment ions of N-acetylanthranillic acid derivatives were m/z119， m/z181， m/z317and m/z359； the characteristic fragment ions of anthranilic acid derivatives were m/z119， m/z181and m/z317； the characteristic fragment ions of phenylacetic acid derivatives were m/z91， m/z181and m/z316. LODs of these three compounds （S/N=3 ∶ 1） were 3-12ng. Conclusion The method is suitable for the identification of N-acetylanthranillic acid， anthranilic acid and phenylacetic acid.