Abstract: Objective Mitochondrial DNA sequencecomparison was performed using shareware Chromas,SeqVerter, Clustal X and GeneDoc. Method mtDNA sequencesof biological samples were automatically analyzed by the ABIPrism Sequencing Analysis Software (V.2.1). All thechromatogram files were opened using Chromas and mergedwith Anderson's reference sequence (.txt) into one multiplesequence file (.txt) using SeqVerter. After inputting the sequences(.aln) and selecting DO COMPLTET ALIGNMENT option inClustal X, The alignment was displayed. The result could thenbe saved as output formats GCG/MSF and printed usingGeneDoc. Conclusion The shareware (Chromas, SeqVerter,Clustal X and GeneDoc) provided an integrated environmentfor multiple sequence alignments and was suitable for mtDNAanalysis in forensic identification.[

Key words: shareware, Chromas, SeqVerter, ClustalX, mtDNA

摘要： 目的 对多个样本的线粒体DNA(mtDNA)高变区测序结果与Anderson标准序列进行比对分析。方法 利用ABI测序仪测定生物学样本的mtDNA高变区序列,得到测序结果文件,通过Chromas、 SeqVerter软件将之转换为aln文件,用ClustalX软件与Anderson标准序列(txt文件)进行比对,确定突变点的碱基排列次序和位置。结果Chromas、SeqVerter和ClustalX软件界面友好,操作简便,可以方便地用于多个样本DNA序列的比较,结果直观,易于判读。结论　运用Chromas、SeqVerter和ClustalX等共享软件,可成功地对多个样本的mtDNA高变区序列进行比对分析。

关键词: 生物学软件, Chromas, SeqVerter, ClustalX, mtDNA