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The Use of Shareware for mtDNA Sequence Comparison
LI Li;LIN Yuan;LIU Yan;ZENG Er-liang;LI Cheng-tao(Institute of forensic sciences;ministry of justice;. United GeneCompany of Shanghai)[
2003(1):
29-31.
Objective Mitochondrial DNA sequencecomparison was performed using shareware Chromas,SeqVerter, Clustal X and GeneDoc. Method mtDNA sequencesof biological samples were automatically analyzed by the ABIPrism Sequencing Analysis Software (V.2.1). All thechromatogram files were opened using Chromas and mergedwith Anderson's reference sequence (.txt) into one multiplesequence file (.txt) using SeqVerter. After inputting the sequences(.aln) and selecting DO COMPLTET ALIGNMENT option inClustal X, The alignment was displayed. The result could thenbe saved as output formats GCG/MSF and printed usingGeneDoc. Conclusion The shareware (Chromas, SeqVerter,Clustal X and GeneDoc) provided an integrated environmentfor multiple sequence alignments and was suitable for mtDNAanalysis in forensic identification.[
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