Abstract: Objective To compare the effect of the novel fluorescent cyanoacrylate powder and the cyanoarylate glue- fluorescent staining in developing latent fingerprints on non-porous surface. Methods Fingerprints dealt with the fluorescent cyanoacrylate powder and the cyanoacrylate glue were fumed in the same fuming cabinet in succession. Afterwards, the fluorescent cyanoacrylate powder fumed fingerprints were directly observed under the blue lamp at 450nm wavelength and photographed, while the cyanoacrylate glue fumed fingerprints were stained with rhodamine 6G and BBD solution before being observed and photographed. Results In general, the one-step method using the fluorescent cyanoacrylate powder revealed the same developing effect with the traditional two-step one. There were also some differences between the two methods in the developing effects, depending on the samples’ nature and the fingerprints’ remaining time. Conclusion The new fluorescent cyanoacrylate powder method can be used as a routine approach to develop latent fingerprints on objects with non-porous surface. Except for of some objects, the overall development quality of this method is comparable to what is achieved by the current two-step fuming and dye staining method. Besides, the new process does not involve organic solvents so the cyanoacrylate polymer won’t dissolve and the ridge details won’t be damaged. The new method also avoids improper staining that may wash the ridge away, as well as some possible damages to exfoliated cells and other biological samples. 

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摘要： 目的 对新型荧光502粉末显现非渗透性光滑客体表面潜手印的一步荧光502显现新技术和传统502胶水熏显+荧光染料染色显现手印技术进行比较研究。方法 比较实验研究，对荧光502粉末和502胶水使用同一自动熏显柜先后进行熏显，荧光502粉末熏显结束后直接在蓝光灯下激发进行观察和拍照；502胶水在熏显完成和聚合物完全固化后使用罗丹明6G和BBD溶液进行荧光染色，再进行光致荧光照相。结果 一步熏显和二次染色的显现效果基本相同，但两者的显现效果也因检材性质和遗留时间的不同而存在一些差别。结论 新型荧光502粉末可以作为一种显现非渗透性光滑客体表面上潜手印的常用方法，尽管新方法对某些客体的显现效果并不好，但对大多数客体来说，其显现效果并不弱于二次染色的方法，并且使用新型荧光502粉末进行一步熏显不会引入有机溶剂，不会引起502聚合物的溶解或造成破坏，确保了纹线及其细节特征不被损坏，也避免了染色后因漂洗不当使手印纹线被冲掉的可能，还不会破坏脱落细胞等生物检材。

关键词: font-size: 10.5pt, mso-bidi-font-size: 10.0pt, mso-ascii-font-family: 'Times New Roman', mso-hansi-font-family: 'Times New Roman', mso-bidi-font-family: 'Times New Roman', mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">新型荧光font-size: 10.5pt, mso-bidi-font-size: 10.0pt, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-fareast-font-family: 宋体" lang="EN-US">502font-size: 10.5pt, mso-bidi-font-size: 10.0pt, mso-ascii-font-family: 'Times New Roman', mso-hansi-font-family: 'Times New Roman', mso-bidi-font-family: 'Times New Roman', mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">粉末；一步熏显；二次荧光染色；比较研究；手印显现