Abstract: Objective To establish a rapid method for the determination of tiletamine and etomidate in e-cigarette oil using high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）. Methods The samples were diluted with methanol and filtered through filter membrane. C18 chromatography column（2.1 mm×100 mm, 2 μm） was used as separation column. Gradient elution was performed with the mobile phase of acetonitrile-0.1%（volume fraction） formic acid aqueous solution. The multiple reaction monitoring (MRM) mode and positive ionization was used for the qualitative and quantitative analysis of tiletamine and etomidate. Results Tiletamine had good linear relationship in the concentration range of 0.02-10.00 ng/mL, and etomidate had good linear relationship in the concentration range of 0.05-10.00 ng/mL. The correlation coefficients were greater than 0.99. The limit of detection for tiletamine and etomidate were 0.01 ng/mL and   0.02 ng/mL, respectively, and the limit of quantification were 0.02 ng/mL and 0.05 ng/mL, respectively. In the spike recovery experiment, the recovery rate of the samples ranged from 95.81% to 102.05%. Conclusion The method is simple to operate with good reproducibility and can be applied to the qualitative and quantitative detection of trace amounts of tiletamine and etomidate in e-cigarette oil.

Key words:

high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）；tiletamine, etomidate；e-cigarette oil

摘要： 目的 建立一种快速测定电子烟油中替来他明和依托咪酯的高效液相色谱-串联质谱方法。方法 样品经甲醇稀释，经过滤膜过滤后测定。以C18色谱柱（2.1 mm×100 mm，2 μm）为分离柱，以乙腈-0.1%（体积分数）甲酸水溶液为流动相进行梯度洗脱，采用多反应监测正离子模式同时对替来他明和依托咪酯进行定性、定量分析。结果 替来他明和依托咪酯分别在0.02~10.00 ng/mL、0.05~10.00 ng/mL范围内线性关系良好，相关系数均大于0.99。替来他明和依托咪酯的检出限分别为0.01、0.02 ng/mL，定量限分别为0.02、0.05 ng/mL，基质加标回收率为95.81%~102.05%。结论 该方法操作简便、方法重现性好，可应用于电子烟油中痕量替来他明和依托咪酯的定性、定量检测。

关键词: 高效液相色谱-串联质谱法, 替来他明, 依托咪酯, 电子烟油