Analysis of Allele Drop-out in the Application of STRtyper-21G (Plus) Kit in Forensic Genetics

doi:10.3969/j.issn.1671-2072.2024.06.008

Chinese Journal of Forensic Sciences ›› 2024 ›› Issue (6): 63-68.DOI: 10.3969/j.issn.1671-2072.2024.06.008

• Forensic Science • Previous Articles Next Articles

Analysis of Allele Drop-out in the Application of STRtyper-21G (Plus) Kit in Forensic Genetics

MAO Huafen^1,2, XU Tianlong¹, ZHOU Baocheng¹, YIN Ting², TANG Xinxin², WANG Leilei²

1. The Judicial Appraisal Institute of the Third People’s Hospital of Lianyungang, Lianyungang 222000, China;
2. The Central Laboratory of Lianyungang Maternal and Child Health Hospital, Lianyungang 222000, China

Received:2023-12-13 Published:2024-11-15 Online:2024-11-28

STRtyper-21G（Plus）试剂盒在法医物证检测中等位基因丢失现象的研究

毛华芬^1，2，许天龙¹，周保成¹，尹婷²，汤欣欣²，王雷雷²

1. 连云港市第三人民医院司法鉴定所，江苏连云港 222000；2. 连云港市妇幼保健院中心实验室，江苏连云港 222000

通讯作者: 王雷雷（1984—），男，副研究员，博士，主要从事遗传学研究。E-mail：Wangleileiok@qq.com
作者简介:毛华芬（1982—），女，副主任技师，硕士，主要从事法医物证学研究。E-mail：31701698@qq.com
基金资助:
连云港市卫生健康科技项目（202331）；连云港市妇幼健康科研项目（F202308）。

Abstract

Abstract: Objective Analyze the drop-out of alleles in the STRtyper-21G （Plus） kit in forensic genetics application, explore its impact on identification results, and propose solutions. Methods Using the STRtyper-21G （Plus） kit, 20 autosomal short tandem repeat (STR) loci of 2 540 blood stains were directly amplified. Capillary electrophoresis was used to analyze the genotypes of each locus. For samples containing loci that do not in accordance with Mendelian inheritance, the STRtyper-32G kit was used for validation. Results After verification by the STRtyper-32G kit, it was found that 9 individuals in 5 cases had allelic drop-out detected using the STRtyper-21G （Plus） kit, involving three loci：D7S820, D19S433, and D18S51. Conclusion In the practice of forensic genetics, using any detection system for detecting STRs may result in allele drop-out and inaccurate paternity test results. To ensure the accuracy of paternity test results, other detection systems with the same locus can be used for verification.

Key words: forensic genetics, STRtyper-21G (Plus) kit, allele drop-out

摘要： 目的分析STRtyper-21G（Plus）试剂盒在法医物证检测中等位基因的丢失情况，探讨其对鉴定结果的影响及解决方法。方法采用STRtyper-21G（Plus）试剂盒对涉及2 540个案例的血斑直接复合扩增20个常染色体STR基因座后进行毛细管电泳，分析各基因座的等位基因分型。对含有不符合孟德尔遗传定律基因座的样本，采用STRtyper-32G试剂盒进行验证。结果经过STRtyper-32G试剂盒验证，在5个案例中发现9名个体采用STRtyper-21G（Plus）试剂盒检测存在等位基因丢失的情况，涉及D7S820、D19S433和D18S51三个基因座。结论在法医物证鉴定实践中，使用任何检测试剂盒进行短串联重复序列的检测都可能发生等位基因丢失而出现亲子鉴定结果不符合实际情况的现象，为保证亲子鉴定结果的准确性，可采用其他具有相同基因座的检测体系进行验证。

关键词: 法医物证, STRtyper-21G（Plus）试剂盒, 等位基因丢失

CLC Number:

DF795.2

MAO Huafen, XU Tianlong, ZHOU Baocheng, YIN Ting, TANG Xinxin, WANG Leilei. Analysis of Allele Drop-out in the Application of STRtyper-21G (Plus) Kit in Forensic Genetics[J]. Chinese Journal of Forensic Sciences, 2024(6): 63-68.

毛华芬, 许天龙, 周保成, 尹婷, 汤欣欣, 王雷雷. STRtyper-21G（Plus）试剂盒在法医物证检测中等位基因丢失现象的研究[J]. 中国司法鉴定, 2024(6): 63-68.

References

[ 1 ] 王乾，刘莹. D10S1248基因座等位基因丢失亲子鉴定1例[J]. 中国法医学杂志，2020，35（6）： 674-675.
[ 2 ] 韩晓龙，刘宏，徐曲毅，等. D18S51基因座等位基因侧翼序列四碱基缺失3例[J]. 刑事技术，2023，48（3）： 324-326.
[ 3 ] 武红艳，张林，樊爱英，等. 导致D5S818等位基因丢失的突变确认1例[J]. 中国法医学杂志，2021，36（3）： 328-329.
[ 4 ] 何丽仪，车志文. 亲子鉴定中STR基因座的等位基因丢失分析与解决对策[J]. 医学与法学，2022，14（3）： 98-102.
[ 5 ] 林汉光，董建国，钟思婷，等. 引物结合区点突变致D18S51等位基因丢失[J]. 刑事技术，2020，45（2）： 201-203.
[ 6 ] 谢素梅，张贵芹，李学博. 亲子鉴定中D18S51基因座等位基因丢失一例之原因分析[J]. 医学与法学，2014，6（1）： 89-90.
[ 7 ] JIANG L R，SONG M X，WANG Z F，et al. Large fragment Sanger sequencing identifies the newly encountered variant that caused null alleles in parentage testing[J]. International Journal of Legal Medicine，2023，137（1）： 57-61.
[ 8 ] HILL C R，KLINE M C，MULERO J J，et al. Concordance study between the AmpFlSTR MiniFiler PCR amplification kit and conventional STR typing kits[J]. Journal of Forensic Sciences，2007，52（4）： 870-873.
[ 9 ] YAO Y N，YANG Q R，SHAO C C，et al. Null alleles and sequence variations at primer binding sites of STR loci within multiplex typing systems[J]. Legal Medicine，2018，30： 10-13.
[10] 刘志勇，任贺，张晓梦，等. 基于有限突变模型和大规模数据的19个常染色体STR的实际突变率研究[J]. 遗传，2021，43（10）： 949-961.
[11] 任静妮，赵珍敏，喻芳，等. D2S1338基因座引物结合区突变致等位基因丢失一例[J]. 中国法医学杂志，2018，33（6）： 635-636.
[12] 陈芳章，陈建红，马武军. 疑难单亲亲子鉴定1例[J]. 中国司法鉴定，2015 （5）： 121-123.

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Analysis of Allele Drop-out in the Application of STRtyper-21G (Plus) Kit in Forensic Genetics

STRtyper-21G（Plus）试剂盒在法医物证检测中等位基因丢失现象的研究

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