Abstract: Objective To investigate the genetic polymorphisms of 15 short tandem repeat （STR） loci in 600 Yunnan Zhuang population, and to explore the genetic characteristics of Zhuang population in Yunnan and its application value in forensic science. Methods AmpFLSTR?Identifiler?Direct PCR Amplification Kit was used for PCR amplification of blood samples from 600 Zhuang-independent individuals in this region. Allele frequencies and forensic parameters（observation of heterozygosity, expected heterozygosity, individual identification Rate, polymorphic information content） were calculated using Modified Powerstats software , using Arlequin 3.5 software to test the Hardy-Weinberg equilibrium and linkage disequilibrium of each locus, and then compare the genetic distance of other groups. Results The discriminative power （DP） values of various Zyx loci in Yunnan were distributed between 0.7790 and 0.9744. The cumulative individual recognition probability （CDP） reached 0.999 999 999 999 999 991 56. The non-parent exclusion rate ranged from 0.2869 to 0.7213. The genetic distance between Yunnan Zhuang and Guizhou Buyi, Guizhou Yi, Guangxi Maonan and Guangxi Dai is relatively close. Conclusion The 15 STR loci of this kit are highly polymorphic in Yunnan Zhuang population, which can be used for forensic identification and paternity identification. It can also be used for group genetics ethnic migration, linguistic branching, and sociological customs. whice can Provide basic research data.

Key words: forensic genetics; genetic polymorphism; STR; Yunnan Zhuang

摘要： 目的 对600个云南壮族人群15个常染色体短串联重复（short tandem repeat，STR）序列基因座的遗传多态性进行调查，探讨云南壮族群体遗传学特性及在法医学中的应用价值。方法 使用AmpFLSTR?Identifiler?Direct PCR扩增试剂盒对此地区600名壮族无关个体血样DNA进行PCR扩增，采用Modified Powerstats软件计算等位基因频率及法医学参数（观察杂合度、期望杂合度、个体识别率、多态信息含量），应用Arlequin 3.5 软件检验各基因座Hardy-Weinberg平衡及连锁不平衡，再比较其他群体的遗传距离。结果 云南壮族各基因座个体识别能力（DP）值分布在0.7790~0.9744之间，累计个体识别概率（CDP）达到0.999 999 999 999 999 991 56，非父排除率值范围在0.2869~0.7213，云南壮族与贵州布依族、贵州侗族、广西毛南族、广西仫佬族遗传距离比较接近。结论 该试剂盒的15个STR基因座在云南壮族人群中具有高度的多态性，可供法医学个体识别和亲权鉴定，亦可为群体遗传学种族迁徙、语言学语言分支区分、社会学风俗探究等提供基础性研究数据。

关键词: 法医遗传学；遗传多态性；STR；云南壮族