Abstract: Objective To seek a new way of estimating postmortem interval (PMI). Methods Comparing obscurity was used to measure the two enzymes' activity of HBDH and LDH in vitreous humor of rabbits under different ambient temperatures (25-30℃;10-15℃) in the course of 0-54 hours after death. Results There was a different plateau period for the two enzymes' activity after a short period of death and the activity decreased quickly after the plateau period, reaching zero after 54 hours of death in low temperature groups and 48 hours in high ones. There existed high negative correlativity between the two enzymes' activity and PMI at a given range (P<0.05). Conclusion PMl can be roughly estimated by using simple regression equation and accurately estimated by using multiple regression equation.

Key words: postmortem interval (PMI), vitreous humor, enzyme 's activity, rabbit

摘要： 目的 寻找一种推断PMI的新方法。方法 应用全自动生化检测仪(用比色法)检测家兔死后0-54小时玻璃体液内羟丁酸脱氢酶(HBDH)、乳酸脱氢酶(LDH)在两组温度下(25-30℃:10-15℃)的失活情况。结果 两种酶的活性在死后一定时间内各出现一个平台期,平台期后两酶的活性迅速下降,低温组在死亡54小时后活性几乎为零,高温组在死亡48小时后活性几乎为零。经统计学分析得知死后两种酶活性与PMI之间有显著的负相关性(P<0.05)。结论 在0-54小时内依据其各自的回归方程将可以大致地推断PMI,依据两种酶活性的多元回归方程可以较准确地推断PMI。

关键词: 死后经过时间, 玻璃体液, 酶活性, HBDH, LDH, 家兔