Abstract: Objective To establish a screening system for simultaneous detection of drugs and toxic compounds in blood by LC-MS/MS. Methods Identification was based on the compound's ab-solute retention time, protonated molecular ion,and one representative fragment ion obtained by multiple reaction monitoring (MRM). The mass spectra library and criteria for identification were established. Results The MRM technique offers high sensitivity of SIS and structural information of scan,and low limits of detection (≤1ng/mL) for majority of the compounds (83%). Conclusion The method is sufficiently selective and sensitive in detecting the drugs down to therapeutic concentration levels and is therefore feasible in forensic and clinical toxicology.The method may also be adapted to other biological samples.

Key words: drugs, screening system, LC-MS/MS, multiplereaction monitoring

摘要： 目的建立血液中常见毒药物的LC-MS/MS筛选分析方法。方法采用多反应监测(MRM)模式,以目标物的保留时间和母离子／子离子对为指标进行筛选分析。建立LC-MS/MS 库和定性认定准则,并考察了方法的有效性。结果多反应监测筛选体系兼具有选择离子扫描的曼敏度和二级质谱的特征性,血液中132种毒药物筛选分析的最低检出限为0．1- 10ng/mL．其中83％的检出限≤lng/mL。鉴定应用表明方法的检出率和可靠性良好。结论该筛选分析方法能满足法医毒物学和临床毒物学检测中毒或治疗浓度目标物的需要,且能扩展至其它生物检材。

关键词: 毒药物, 筛选分析, LC-NS／MS, 多反应监测