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2006 Issue 01 (Total No. )
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Screening for 132 Drugs in Blood by LC-MS/MS with Multiple-Reaction Monitoring SHEN Min;XIANG Ping;SHEN Bao-hua;MA Dong;YAN Hui;WANG Meng-ye(Institute of Forensic Sciences;Ministry of Justice;Shanghai 000;China) 2006(01): 14-20.  Abstract ( )   Objective To establish a screening system for simultaneous detection of drugs and toxic compounds in blood by LC-MS/MS. Methods Identification was based on the compound's ab-solute retention time, protonated molecular ion,and one representative fragment ion obtained by multiple reaction monitoring (MRM). The mass spectra library and criteria for identification were established. Results The MRM technique offers high sensitivity of SIS and structural information of scan,and low limits of detection (≤1ng/mL) for majority of the compounds (83%). Conclusion The method is sufficiently selective and sensitive in detecting the drugs down to therapeutic concentration levels and is therefore feasible in forensic and clinical toxicology.The method may also be adapted to other biological samples. Related Articles | Metrics

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Y-STR Genotyping in Forensic Identification Using a 17-Ioci Multiplex PCR Assay LI Cheng-tao;LI Li;LIN Yuan;LIU Yan;QUE Ting-zhi;ZHAO Zhen-min(Institute of Forensic Sciences;Ministry of Justice;shanghai 000;China) 2006(01): 21-23.  Abstract ( )   Genotyping of Y-STR loci plays an important role in the field of forensic identification.A variety of commercial Y-STR kits have been exploited. Recently, a novel Y-STR genotyping kit named Yfiler come onto the market. It contains 17 Y-STR loci and the loci can be co-amplified in a single tube.The kit's validation was studied through a practical case. Related Articles | Metrics