Abstract: Objective To establish a method for determination of barbiturate drugs in blood. Methods Barbiturate drugs in blood were extracted with organic solvent and detected by LC-MS/MS with aspirin internal standard. Results The limits of detection (LOD) were 10ng/mL for all the 5 barbiturate drugs, the calibration range 0.02 (10μg/mL), the recovery rate 70 (87%). Conclusion The method is sensitive, accurate and reliable. It is shown to be applicable for detecting barbiturate drugs in forensic and clinical analytical toxicology.

Key words: barbiturate drug, blood, LC-MS/MS

摘要： 目的建立血液中巴比妥类药物液相色谱-串联质谱(LC-MS/MS)检测方法。方法血液中的巴比妥类药物用有机溶剂液-液萃取,以乙酰水杨酸为内标,LC-MS/MS测定。结果检测血液中五种巴比妥类药物的最小检出限(LOD)均为10ng/mL,线性范围在0.02￣10μg/mL内良好,方法的回收率70%￣87%。结论所建方法快速、灵敏、准确,可以满足临床毒物和法庭毒物分析的需要。

关键词: 巴比妥类药物, 血液, 液相色谱-串联质谱(LC-MS/MS)