Application and Evalution of Identifiler and Sinofiler Kit in Forensic Paternity Testing

Abstract

Abstract: objective To apply and evaluate two different kits-- Identifiler and Sinofiler in paternity testing of Chinese Han population. Methods By multiplex amplification system, capillary electrophoresis and GeneMapper typing, 15 STR loci were genotyped in 106 cases of paternity testing. Results In inclusive cases, Sinofiler has higher PI valves than Identifiler; in exclusive cases, Sinofiler is more effective than Identifiler. Conclusion The results show that Sinofiler is more suitable in Chinese Han paternity testing.

Key words: Identifiler kit, Sinofiler kit, TH01, TPOX, D6S1043, D12S391, paternity testing, STR

摘要： 目的应用与评估Identifiler和Sinofiler试剂盒在亲权鉴定中的应用价值。方法应用五色荧光标记复合扩增系统对106例亲权鉴定的血样进行PCR扩增,用ABI3100-Avant遗传分析仪对扩增产物进行毛细管电泳,用GeneMapper v3.2软件进行基因分型,评估Identifiler和Sinofiler试剂盒在中国汉族人群中的适用性。结果在不排除的案例中Sinofiler比Identifiler具有较高的PI值,在排除的案例中Sinofiler比Identifiler具有较多的排除指标。结论Sinofiler比Identifiler更适用于中国汉族人群的亲权鉴定。

关键词: Identifiler试剂盒, Sinofiler试剂盒, TH01, TPOX, D6S1043, D12S391, 亲权鉴定, 短串联重复序列

LIN Yuan;GUO Hong;ZHAO Zhen-ming;LI Li(. Institute of Forensic Science;Ministry of Justice;Shanghai 000;China;.Medical School of Soochow University;Suzhou;China). Application and Evalution of Identifiler and Sinofiler Kit in Forensic Paternity Testing[J]. , 2008(01): 40-41+4.

林源;郭宏;赵珍敏;李莉;. Identifiler和Sinofiler试剂盒在亲权鉴定中的应用与评估[J]. , 2008(01): 40-41+4.

[1]	WANG Minyuan, LI Jia. Analysis on the Dual Structure of Forensic Appraisal Law [J]. Chinese Journal of Forensic Sciences, 2024, 0(1): 1-11.
[2]	BAI Rongjing. Normative Analysis and Procedural Improvement of Supervision and Appraisal Legislation ——Using the Forensic Appraisal System as a Reference [J]. Chinese Journal of Forensic Sciences, 2024, 0(1): 18-27.
[3]	QIAO Fang’e. Introspection and Reconstruction of Self-commissioned Appraisal Opinion [J]. Chinese Journal of Forensic Sciences, 2024, 0(1): 82-90.
[4]	ZHU Jinfeng. Outline of Several Issues on Forensic Appraisal Management under the Public Welfare Attribute [J]. Chinese Journal of Forensic Sciences, 2023, 0(5): 10-21.
[5]	SHEN Min, YAN Hui, XU Duoqi, JI Jiaojiao. Frontier in Hair Analysis：Research Advances in Hair Microsampling Technology [J]. Chinese Journal of Forensic Sciences, 2023, 0(5): 22-30.
[6]	CHEN Dingwen, . Experimental Research on the Handwriting Characteristics of Dehong Dai Script [J]. Chinese Journal of Forensic Sciences, 2023, 0(4): 66-72.
[7]	WEI Wei, YANG Jie, XU Chongchong, LU Xiaoxiao, YUAN Xiaokun, YANG Shuo, ZHANG Shouxun, XIA Sheng, LI Kuan, LIAN Xinqing, ZHONG Shurong, . Study of the Genetic Polymorphism of 20 Autosomal STR Loci in Yi Population in Honghe Region of Yunnan Province [J]. Chinese Journal of Forensic Sciences, 2023, 128(3): 78-85.
[8]	YANG Xiaoli. The Perfection of the Integrity System of Forensic Appraisers from the Perspective of the Connection between the Civil Procedure Law and Forensic Administration Management [J]. Chinese Journal of Forensic Sciences, 2023, 127(2): 12-20.
[9]	LIAO Yi, GAO Bodi, ZHAO Xiaomeng, ZHONG Juanfang, ZHANG Qianjun, DU Juan, TAN Yueqiu, LU Guangxiu, LIN Ge, LI Wen, . Genetic Polymorphism of 20 STR Loci in Hunan Han Population [J]. Chinese Journal of Forensic Sciences, 2023, 127(2): 67-75.
[10]	WANG Lianzhao. On the Legislative Value and Construction Logic of the Forensic Appraisal Law [J]. Chinese Journal of Forensic Sciences, 2023, 126(1): 29-37.
[11]	WU Lina, XIAO Fu, LIN Jiaman, LI Tianle, XIANG Ping, SHI Yan, YUN Keming. Research Progress and Application of QSAR in New Psychoactive Substances [J]. Chinese Journal of Forensic Sciences, 2023, 126(1): 45-52.
[12]	YANG Zihao, JIANG Zhiwei, WU Liming, TAO Ruiyang, XIA Ruocheng, LI Chengtao, DU Zhichun. A Comparative Study of Pretreatment and DNA Extraction Methods for Bones from Different Parts [J]. Chinese Journal of Forensic Sciences, 2023, 126(1): 63-70.
[13]	XUE Nan. System Reconstruction of Forensic Appraisal Assistance [J]. Chinese Journal of Forensic Sciences, 2022, 122(3): 10-17.
[14]	WANG Changliang, LÜ Chen, LU Yan, LIU Xiaoying, GU Yuqing, ZHOU Guanglei, LI Biao. Research of Time Effects on Microscopic Results of Identification #br# of the Sequence between Stamps and Handwriting#br# [J]. Chinese Journal of Forensic Sciences, 2022, 122(3): 63-69.
[15]	XIA Ruocheng, TAO Ruiyang, LIN Yuan, ZHANG Xiaochun, YANG Qi, YU Huan, XI Shihan, XIONG Lei, LI Chengtao, ZHANG Suhua. Research Progress on Forensic Application of Cannabis sativa Genetic Markers [J]. Chinese Journal of Forensic Sciences, 2022, 120(1): 66-72.

Application and Evalution of Identifiler and Sinofiler Kit in Forensic Paternity Testing

Identifiler和Sinofiler试剂盒在亲权鉴定中的应用与评估

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics