Abstract: Objective A new identification technology was established through desorption electrospray ionization （DESI）-Time of Flight Mass spectrometry imaging（TOFMS-HDI） technology in this paper. Methods 90 samples of “11” prepared by 10 black signature pens to “47” were tested. Results The original handwriting “11” could be showed in two-dimensional images. Conclusion DESI MS-HDI technology is an effective document inspection technique without the need for sample pretreatment, which is easily used under the atmospheric pressure and non-destructive inspection and suitable for document identification and tampering with document identification.

Key words: DESI; molecular imaging; HRMS

摘要： 目的 通过电喷雾解吸电离（DESI）—高分辨飞行时间质谱（HRMS-HDI）成像技术建立了一种新型添改字迹鉴别技术。方法 对10支黑色签字笔书写的“11”被添改成“47”的90个样本用分子成像技术测试。结果 该技术可将84个被添改的原始字迹“11”以二维成像方式显现。结论 DESI-HRMS-HDI技术无需检材预处理，在大气压下即可进行操作，接近无损检验，操作简便，是一种有效的文件检验技术，适用于文件材料鉴别和篡改文件鉴定。

关键词: 电喷雾解吸电离；高分辨质谱；分子成像；篡改文件鉴定