Abstract: Objective To establish a determination method of ultra-high performance liquid chromatography-tandem mass spectrometry （UHPLC-MS/MS） for simultaneous analysis of 8 phencyclidine-type substances in blood. Methods Processing blood using acetonitrile protein precipitation method, use an ACQUITY UPLC BEH C18 column （2.1 mm×100 mm, 1.7 μm）, and select acetonitrile-water containing 0.1% （volume fraction） formic acid as the mobile phase at 0.4 mL/min. Mass spectrometry was detected using an electrospray ionization-positive ion, dynamic multiple reaction monitoring （MRM） detection mode. Results This method achieved qualitative and quantitative analysis of 8 phencyclidine-type substances in blood. Each target object has a good linear relationship in the range of 0.1-50 ng/mL, with the correlation coefficients all greater than 0.997. The minimum detection limits range from 0.015 ng/mL to 0.03 ng/mL, and the minimum quantitative limits ranged from 0.05 ng/mL to 0.1 ng/mL. The proposed method was successfully applied to actual blood and detected the 2-FXE component in the blood. Conclusion This method is rapid, sensitive, accurate and has wide applicability, which can meet the requirements of qualitative and quantitative analysis of phencyclidine-type substances in blood. It can also provide a reference for analyzing new phencyclidine-type substances in the future.

Key words: phencyclidine-type substance, blood, acetonitrile protein precipitation method, UHPLC-MS/MS

摘要： 目的 建立同时测定血液中8种苯环利定（phencyclidine，PCP）类物质的超高效液相色谱-串联质谱检测方法。方法 使用乙腈蛋白沉淀法处理血液，采用ACQUITY UPLC BEH C18 色谱柱（2.1 mm×100 mm，1.7 μm），以0.1%（体积分数）甲酸溶液-含0.1%甲酸溶液的乙腈为流动相进行梯度洗脱，流速 0.4 mL/min。质谱检测采用电喷雾离子源正离子、动态多反应监测模式。结果 该方法实现了对血液中8种苯环利定类物质的定性、定量分析，各目标物在0.1~50 ng/mL范围内具有良好的线性关系，相关系数均大于0.997，检出限为0.015~0.03 ng/mL，定量限为0.05~0.1 ng/mL。该方法成功应用于实际血液，于该血液中检出2-FXE成分。结论 该方法具有快速、灵敏、准确、适用性广等突出特点，能够满足血液中苯环利定类物质的定性、定量分析要求，并为分析未来新出现的苯环利定类物质提供参考。

关键词: 苯环利定类物质, 血液, 乙腈蛋白沉淀法, 超高效液相色谱-串联质谱法