Fingerprint has always been an indispensable personal identification tool in court scientific investigation. The fingerprint not only carries the morphological information of the fingerprint ridge, but also provides the chemical information of the endogenous and exogenous substances of the fingerprint, which can reflect the personal characteristics of the provider, such as age, gender, race and possible drug abuse habits. With the development of analytical instruments, the residual substances in fingerprints can be clearly presented by imaging technology. This paper introduces the application of spectroscopy, mass spectrometry and immunoassay technology in the identification of fingerprint residues in forensic science, and discusses the advantages and disadvantages of each method. In practice, the appropriate technology can be selected according to the specific situation. In the future, imaging technology will have new development in sensitivity and specificity, spatial resolution, quantitative stability and on-site rapid analysis, so as to better serve the investigation of various cases.