Objective CircRNAs in human skeletal muscle with temporal changes in death time were screened by constructing circular RNA (circRNA) expression profiles in skeletal muscle. The selected circRNAs were further verified, and then the correlation between the relative expression and the time of death was investigated. Methods T In the chip experiment, 2 individuals with relatively uniform external temperature and humidity, and known to have a death time of less than 24 hours, were taken for 50 g each at the autopsy (within 12 hours), 3 days, 5 days, 7 days, and 10 days. The RNA was extracted, and then reverse transcription, probe labeling, and gene chip detection were performed to obtain the expression profile of circRNA. The selected circRNAs were verified in 18 samples collected in the subsequent samples, and the obtained data were statistically analyzed. Results A total of 13156 circRNAs were detected in skeletal muscle tissue, but the total number of circRNAs that showed a downward trend within 10 days was only hsa_circ_0001727, hsa_circ_0001136, hsa_circ_0001871, hsa_circ_0005251, hsa_circ_0000284, hsa_circ_0000520, hsa_circ_0001971 and so on. The 7 circRNAs screened were verified in 18 samples, and only 5 circRNAs such as hsa_circ_0001727, hsa_circ_0001136, hsa_circ_0001871, hsa_circ_0005251, hsa_circ_0000284, etc., with the increase of death time, the relative expression decreased significantly and statistically (P<0.05). The expression of two circRNAs, hsa_circ_0000520 and hsa_circ _0001971, was negative. Conclusion circRNA can be used as a more stable biomarker for the study of death time inference.