主管：中华人民共和国司法部
主办：司法鉴定科学研究院
ISSN 1671-2072  CN 31-1863/N
中国司法鉴定

中国司法鉴定 ›› 2025 ›› Issue (2): 47-52.DOI: 10.3969/j.issn.1671-2072.2025.02.006

• 鉴定科学 • 上一篇    下一篇

合成大麻素ADB-BUTINACA在豚鼠体内的药代动力学研究

张佳丽1，2，向  平1，王新颖1，3，王  鑫1   

  1. 1.司法鉴定科学研究院 上海市法医学重点实验室 上海市司法鉴定专业技术服务平台 司法部司法鉴定重点实验室，上海 200063； 2.山西医科大学 法医学院，山西 晋中 0306003；3.沈阳药科大学 药学院，辽宁 沈阳 110016
  • 收稿日期:2024-05-07 出版日期:2025-03-15 发布日期:2025-03-25
  • 作者简介:张佳丽（1998—），女，硕士研究生，主要从事法医毒物分析。E-mail：zhangjiali310@163.com
  • 基金资助:
    “十四五”国家重点研发计划（2022YFC3300903）；国家自然科学基金资助项目（82002000）；上海市法医学重点实验室资助项目（21DZ2270800）；上海市司法鉴定专业技术服务平台资助项目；司法部司法鉴定重点实验室资助项目

Pharmacokinetic Study of Synthetic Cannabinoid ADB-BUTINACA in Guinea Pigs

ZHANG Jiali1, 2, XIANG Ping1, WANG Xinying1, 3,  WANG Xin1   

  1. 1. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Key Laboratory of Forensic Science, Ministry of Justice, Academy of Forensic Science, Shanghai 200063, China； 2. School of Forensic Medicine, Shanxi Medical University, Jinzhong 030600, China；3. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
  • Received:2024-05-07 Published:2025-03-15 Online:2025-03-25

PDF

摘要： 目的 探讨单次给药后豚鼠体内ADB-BUTINACA的代谢动力学特征。方法 取16只豚鼠随机分为低剂量组（0.1 mg/kg）和高剂量组（0.5 mg/kg），腹腔注射给药ADB-BUTINACA，采用液相色谱-串联质谱法测定不同时间点（0.02、0.08、0.16、0.25、0.33、0.5、0.66、1、2、4、6、12、24 h）的血液和12~24 h的尿液中ADB-BUTINACA和代谢物ADB-BUTINACA acid的含量，采用药代动力学软件计算药动学参数。结果 单次给药后，血液中ADB-BUTINACA迅速达峰并很快消除，ADB-BUTINACA acid含量低于定量限。低、高剂量组血液中ADB-BUTINACA的Tmax分别为20.00、23.75 min，Cmax分别为11.42、21.37 ng/mL，T1/2分别为33.85、34.14 min。给药后12~24 h的尿液中检出代谢物，未检出原体。结论 ADB-BUTINACA在豚鼠体内的吸收和消除速率均较快，给药后20 min左右血液浓度达到峰值，2 h后不可测，检测时限较短，而尿液检测时限可至12~24 h。该研究可为ADB-BUTINACA的相关毒理学研究和鉴定实践提供参考数据。

关键词: ADB-BUTINACA, 液相色谱-串联质谱法, 血药浓度, 代谢动力学

Abstract: Objective To investigate the pharmacokinetics of ADB-BUTINACA in guinea pigs after a single administration. Methods Sixteen guinea pigs were randomly divided into low-dose (0.l mg/kg) and high-dose (0.5 mg/kg) groups, and ADB-BUTINACA were administered intraperitoneally. The concentrations of ADB-BUTINACA and its metabolite ADB-BUTINACA acid were determined in blood samples collected at various time points (0.02, 0.08, 0.16, 0.25, 0.33, 0.5, 0.66, 1, 2, 4, 6, 12, and 24 h), and in urine samples collected from 12 h to 24 h post-dosing using liquid chromatography-tandem mass spectrometry（LC-MS/MS）. Pharmacokinetic parameters were calculated using pharmacokinetic software. Results After single doses, ADB-BUTINACA rapidly reached its peak concentration in blood and was quickly eliminated, while the concentration of ADB-BUTINACA acid remained below the limit of quantification. The Tmax values for ADB-BUTINACA in blood were 20.00 min and 23.75 min for the low-dose and high-dose groups, respectively. The Cmax values were 11.42 ng/mL and 21.37 ng/mL, respectively, and the T1/2 values were 33.85 min and 34.14 min, respectively. The metabolite was detected in urine samples collected from 12 h to 24 h post-dosing, while the parent compound was not detected. Conclusion The results of this study indicate that  ADB-BUTINACA is rapidly absorbed and eliminated in guinea pigs, with peak blood concentrations occurring approximately 20 min after administration and becoming undetectable after 2 h, suggesting a short detection window. While the detection window of urine can be extended to be 12 h to 24 h. This study provides reference data for toxicological research and forensic identification practices involving ADB-BUTINACA.

Key words: ADB-BUTINACA, LC-MS/MS, blood concentration, pharmacokinetics

中图分类号: