Abstract:

Objective To establish a HPLC method for determination of 2，4-D butyl ester in blood and urine. Methods Compound was extracted from blood and urine using ethyl acetate. Separation was achieved on Zorbax SB-Aq column using methanol-water（60 ∶ 40） as mobile phase. Results The calibration curve of 2，4-D butyl ester ranged from 0.10 μg/mL to 10.00 μg/mL in blood （ r ≥0. 999 8） and 0.08 μg/mL to 8.00 μg/mL in urine （r≥0.999 5）， respectively. The limits of detection for the analyte was 0.002 0 μg/mL and 0.001 8 μg/mL in blood and urine， respectively. The intra and inter day assay precision were no more than 4.5%. The mean recovery ranged from 94.5% to 104.5%. Conclusions The pre-treatment and HPLC analysis of 2，4-D butyl ester in blood and urine can be used for rapid diagnosis of 2，4-D butyl ester poisoning and forensic identification of 2，4-D butyl ester poisoning death.

Key words: 2, 4-D butyl ester, HPLC, forensic toxicological analysis, biological specimen

摘要：

目的 建立检测血液、尿液中2，4-D丁酯的高效液相色谱分析方法。方法 采用正己烷为样品萃取溶剂，色谱柱为Zorbax SB - Aq柱，流动相为V（甲醇） ∶ V（水） ＝ 60 ∶ 40。结果 2，4-D丁酯在血液和尿液中的线性范围分别为0.10～10.00 μg/mL （ r≥0. 999 8）和0.08～8.00 μg/mL （ r≥0.999 5），检测限分别为0.002 0 μg/mL和0.001 8 μg/mL，准确度为94.5% ～104.5%，日内、日间精密度≤4.5% 。结论 本研究建立的血液、尿液中2，4-D丁酯的提取和HPLC检测方法，可应用于2，4-D丁酯中毒的快速检验和中毒死亡的法医学鉴定。

关键词: 2, 4-D丁酯, 高效液相色谱法, 法医毒物分析, 生物样品