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Study on Self-generation Alcohol in Preserved Dog Corpses
LIU Xiao-xia;QIAO Jun-yuan;YUN Ke-ming;ZHANG Da-ming;WEI Zhi-wen;AN jian-kang;WANG Yu-jin (. Forensic Medicine School of Shanxi Medical University;Taiyuan 0000;China;. Xinghualing Branch of Taiyuan Public Security Bureau;Taiyuan 0000;China
2010(01):
26-29.
Objective To establish an analysis of alcohol and self-generation alcohol in biological samples by a direct-injection gas chromatogram; to develop an animal model for self-generation alcohol research and observe self-generation alcohol in pre-served dog corpses. Methods After executed by CO2,dogs were stored at room temperature and were sampled in 0,2,4,8,12,24,48,72,96,120h. The samples were homogenated and diluted (1:2 for tissue homogenate,1:4 for body fluid) with the inter-nal standard solution. Using a direct-injection gas chromatogram,the alcohol and self-generation alcohol in the samples were analyzed qualitatively and quantitatively by the retention time and internal standard method. Results The retention times of methanol,acetone,alcohol,n-propyl alcohol,isobutanol,isopropanol,n-butanol,isoamyl alcohol were (2.77±0.01)min,(3.42± 0.01)min,(3.87±0.01)min,(5.92±0.01)min,(7.54±0.01)min,(7.92±0.01)min,(10.33±0.01)min,(13.55±0.01)min,respec-tively. Alcohol was detected in heart-blood,inferior vena cava blood,urine,bile,liver,spleen,lung,kidney in 12h after death,and in muscle,humor vitreous,brain in 48,72,96h. There was a positive correlation between alcohol and n-propyl alcohol. Conclusion Alcohol can be produced postmortemly in dog corpses. Alcohol detected in urine can not be thought as the marker of antemortem drinking. In forensic identification of alcohol-related cases,self-generation alcohol should be taken into consid-eration. The unrotten tissues such as muscle,humor vitreous and brain should be sampled for the determination of alcohol and self-generation alcohol,and the ratio of alcohol and n-propyl alcohol in samples could be used for identification of antemortem drinking.
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